Structural and functional multiplicity of the kisspeptin/GPR54 system in goldfish (Carassius auratus)
Structural and functional multiplicity of the kisspeptin/GPR54 system in goldfish (Carassius auratus)
复制标题
金鱼 (Carassius auratus) 中 Kisspeptin/GPR54 系统的结构和功能多样性
DOI：
10.1677/joe-09-0016
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发表时间：
2009-06-01
影响因子：
4
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Li, Shuisheng;Zhang, Yong;Lin, Haoran
To ascertain the neuroendocrine function of the kisspeptin/GPR54 system in non-mammalian species, full-length cDNAs encoding for Kiss1 and Kiss2 as well as their putative cognate receptors GPR54a and GFR54b, were isolated from goldfish (Carassius auratus). The deduced protein sequences between Kiss1 and Kiss2 in goldfish share very low similarity, but their putative mature peptides (kisspeptin-10) are relatively conserved. RT-PCR analysis demonstrated that the goldfish kiss1 gene (gfkiss1) is highly expressed in the optic tectum-thalamus, intestine, kidney, and testis, while the goldfish kiss2 gene (gfkiss2) is mainly detected in the hypothalamus, telencephalon optic tectum thalamus, adipose tissue, kidney, heart, and gonads. The two receptor gene, (gfgpr54a and gfgpr54b) are highly expressed in the brain regions including telencephalon, optic tectum, thalamus, and hypothalamus. Both mature goldfish kisspeptin-10 peptides (gfKiss1-10 and gfKiss2-10),ire biologically active as they could functionally interact with the two goldfish receptors expressed in cultured eukaryotic cells to trigger the downstream signaling pathways with different potencies. The actions of gfKiss1-10 and gfKiss2-10 oil LH secretion were further investigated in vitro and fit vivo. Intraperitoneal administration of gfKiss1-10 to Sexually mature female goldfish could increase the serum LH levels. However, this peptide does not significantly influence LH release from goldfish pituitary cells in primary culture, indicating that the peptide does not exert its actions at the pituitary level. Oil the other hand, gfKiss2-10 appears to be a much less potent peptide is it exhibits no significant in vivo bioactivity and is also inactive oil the primary pituitary cells. journal of Endocrinology (2009) 201, 407-418
