ENZYME-IMMUNOASSAY - AN IMPROVED DETERMINATION OF URINARY ALBUMIN IN DIABETICS WITH INCIPIENT NEPHROPATHY
ENZYME-IMMUNOASSAY - AN IMPROVED DETERMINATION OF URINARY ALBUMIN IN DIABETICS WITH INCIPIENT NEPHROPATHY
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DOI：
10.3109/00365518509155256
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发表时间：
1985-01-01
影响因子：
2.1
通讯作者：
DECKERT, M
中科院分区：
文献类型：
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作者：
FELDTRASMUSSEN, B;DINESEN, B;DECKERT, M
一种酶联免疫吸附测定尿白蛋白使用市售试剂描述。测定范围为2.5-120 μ g/1。当样品以两种标准稀释度进行分析时，可检测的白蛋白浓度范围为2.5- 240mg /l，涵盖了从正常蛋白尿到明显临床肾病的临床范围。测定内变异为2.1%，测定间变异为8.3%。尿液中添加白蛋白的回收率为95% ~ 106%，尿液稀释度呈线性。免疫扩散检测与尿白蛋白的相关性极好（n=80, r=0.99）。早期糖尿病肾病患者不同天数24小时尿白蛋白排泄量的个体内变异较高（51.5%），考虑肌酐排泄量的变异后个体内变异略有降低（39.5%）。白蛋白排泄与HbA1c或尿糖排泄之间没有相关性，这表明微小的代谢变化并不是导致UalbV在个体内巨大的日常变化的原因。研究表明，在将患者分为有或没有早期糖尿病肾病的组之前，必须进行不止一次的UalbV测量。
An enzyme linked immunoadsorbent assay for urinary albumin using commercially available reagents is described. The assay range is 2.5-120 .mu.g/1. When samples are analysed in two standard dilutions, the assayable albumin concentration range is 2.5-240 mg/l, covering the clinical range from normoalbuminuria to overt clinical nephropathy. Intra-assay variation was 2.1% and interassay variation 8.3%. Recovery of added albumin to urine was 95%-106% and dilution of urine was linear. The correlation to urinary albumin determined by immunodiffusion was excellent (n=80, r=0.99). Intraindividual variation of the 24 h urine albumin excretion of different days was high in patients with incipient diabetic nephropathy (51.5%) and was only slightly reduced by taking the variation of creatinine excretion into account (39.5%). No correlation was found between albumin excretion, and HbA1c or urine glucose excretion, indicating that minor metabolic variations are not responsible for the huge intraindividual day-to-day variations of UalbV. The study shows that more than one UalbV measurement must be done before classifying patients into groups with or without incipient diabetic nephropathy.