Genetic and transcriptomic analyses of lignin- and lodging-related traits in Brassica napus
Genetic and transcriptomic analyses of lignin- and lodging-related traits in Brassica napus
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甘蓝型油菜木质素和倒伏相关性状的遗传和转录组分析
DOI：
10.1007/s00122-017-2937-x
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发表时间：
2017
影响因子：
5.4
通讯作者：
Li Jiana
中科院分区：
文献类型：
--
作者：
Wei Lijuan;Jian Hongju;Lu Kun;Yin Nengwen;Wang Jia;Duan Xiujian;Li Wei;Liu Liezhao;Xu Xinfu;Wang Rui;Paterson Andrew H.;Li Jiana
Key messageCandidate genes associated with lignin and lodging traits were identified by combining phenotypic, genotypic, and gene expression data inB. napus.AbstractBrassica napusis one of the world’s most important oilseed crops, but its yield can be dramatically reduced by lodging, bending, and falling of its vertical stems. Lignin has been shown to contribute to stem mechanical strength. In this study, we found that the syringyl/guaiacyl (S/G) monolignol ratio exhibits a significant negative correlation with disease and lodging resistance. A total of 92 and 50 SNP and SSR loci, respectively, were found to be significantly associated with five traits, breaking force, breaking strength, lodging coefficient, acid detergent lignin content, and theS/Gmonolignol ratio using GWAS. To identify novel genes involved in lignin biosynthesis, transcriptome sequencing of high- (H) and low (L)-ADL content accessions was performed. The up-regulated genes were mainly involved in glycoside catabolic processes (especially glucosinolate catabolism) and cell wall biogenesis, while down-regulated genes were involved in glucosinolate biosynthesis, indicating that crosstalk exists between glucosinolate metabolic processes and lignin biosynthesis. Integrating this differential expression with the GWAS analysis, we identified four candidate genes regulating lignin, including glycosyl hydrolase (BnaA01g00480D),CYT1 (BnaA04g22820D), and two encoding transcription factors,SHINE1(ERF family) andDAR6(LIM family). This study provides insight into the genetic control of lodging and lignin inB. napus.
