Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro
Effects of Deferoxamine on the Repair Ability of Dental Pulp Cells In Vitro
复制标题
去铁胺对牙髓细胞体外修复能力的影响
DOI：
10.1016/j.joen.2013.12.016
复制
发表时间：
2014-08-01
影响因子：
4.2
通讯作者：
Zhu, Ya-Qin
中科院分区：
文献类型：
--
作者：
Jiang, Long;Peng, Wei-Wei;Zhu, Ya-Qin
在以往的研究中，我们发现缺氧可以促进牙髓细胞的矿化。然而，临床应用缺氧作为一种治疗是值得怀疑的或不可行的。去铁胺（DFO），一种治疗铁超载的药物，也被证明会导致缺氧。本研究旨在探讨DFO对DPCs修复能力的影响。方法：采用体外组织外植体技术获得DPCs，分别用不同浓度的DFO或缺氧培养2 d。对DPCs的活力、增殖、迁移和牙源性分化进行了检测和分析。Western blotting检测缺氧诱导因子1- α (HIF-1 α)的表达。结果：10微磨牙DFO可增强HIF-1 α的表达，与缺氧相似，但在2天内不影响DPCs的生存能力。此外，DFO还能促进DPCs的增殖、迁移和成牙分化。结论：DFO可能通过HIF-1 α提高DPCs的修复能力。
Introduction: In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. Methods: DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1 alpha) was assessed through Western blotting. Results: Ten micromolars of DFO enhanced the expression of HIF-1 alpha similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. Conclusions: These results suggest that DFO might improve the repair ability of DPCs by HIF-1 alpha.