Rapid Discrimination of Archaeal Tetraether Lipid Cores by Liquid Chromatography-Tandem Mass Spectrometry
Rapid Discrimination of Archaeal Tetraether Lipid Cores by Liquid Chromatography-Tandem Mass Spectrometry
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DOI：
10.1016/j.jasms.2008.09.015
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Keely, Brendan J.
中科院分区：
文献类型：
--
作者：
Knappy, Christopher S.;Chong, James P. J.;Keely, Brendan J.
大气压化学电离液相色谱-串联质谱（APCI LC-MS/MS）的四醚脂质核心的古细菌起源揭示了不同的解离途径的三个类的核心脂质提取的甲烷嗜热自养。在这些类别中，两个同量异序四醚脂质，一个是很少报道的脂质成分的M。热自养菌和另一种在从培养的细胞提取期间形成的人工产物，通过它们的MS 2光谱进行鉴定和区分。APCI LC-MS/MS区分不同的四醚核心脂质类型和同量异序物质，并揭示了四醚核心内的组成联植烷链的质量，尽管没有完全阐明其结构。此外，该方法允许直接估计的相对比例的四醚核心脂质的色谱峰面积测量，允许快速分析这些化合物在微生物和环境提取物。（J Am Soc Mass Spectrom 2009，20，51-59）（C）2009年美国质谱学会
Atmospheric pressure chemical ionization liquid chromatography-tandem mass spectrometry (APCI LC-MS/MS) of tetraether lipid cores of archaeal origin reveals distinct dissociation pathways for three classes of core lipid extracted from Methanobacter thermautotrophicus. Within these classes, two isobaric tetraether lipids, one a scarcely reported lipid constituent of M. thermautotrophicus and the other an artefact formed during extraction from cultured cells, were identified and distinguished via their MS2 spectra. APCI LC-MS/MS discriminates different tetraether core lipid types and isobaric species and reveals the mass of the constituent biphytanyl chains within the tetraether cores, albeit without full elucidation of their structures. Furthemore, the method allows direct estimation of the relative proportions of tetraether core lipids from chromatographic peak area measurement, allowing rapid profiling of these compounds in microbiological and environmental extracts. (J Am Soc Mass Spectrom 2009, 20, 51-59) (C) 2009 American Society for Mass Spectrometry