SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE
SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE
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DOI：
10.1016/0003-2697(80)90165-7
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
PAIGEN, K
中科院分区：
文献类型：
--
作者：
LABARCA, C;PAIGEN, K
描述了一种简单快速的定量测定粗匀浆中 DNA 的方法。该方法基于双苯并咪唑 (Hoechst 33258) 与 DNA 结合时荧光的增强。用高盐缓冲液解离染色质的粗匀浆可以在几分钟内直接、可靠地进行测定。染色质的解离对于使用该方法准确测定生物材料中的 DNA 至关重要。该检测可以使用相当简单的仪器检测低至 10 ng 的 DNA。
A simple and rapid assay for quantitative determinations of DNA in crude homogenates is described. The method is based on the enhancement of fluorescence seen when bisbenzimidazole (Hoechst 33258) binds to DNA. Crude homogenates in which chromatin was dissociated with high salt buffer can be assayed directly and reliably in a few minutes. The dissociation of chromatin is critical to accurate determinations of DNA in biological materials using this method. The assay can detect as little as 10 ng of DNA with rather unsophisticated instrumentation.