Cuticle of Caenorhabditis elegans: its isolation and partial characterization.
Cuticle of Caenorhabditis elegans: its isolation and partial characterization.
复制标题
秀丽隐杆线虫的角质层：其隔离和部分特征。
DOI：
10.1083/jcb.90.1.7
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发表时间：
1981-07
影响因子：
7.8
通讯作者：
Edgar, R S
中科院分区：
文献类型：
--
作者：
Cox, G N;Kusch, M;Edgar, R S
土壤线虫的成体角质层是一种蛋白质类细胞外结构，在动物生命周期的最后一次蜕皮过程中，由底层的皮下细胞阐述。角质层由外部皮质层组成，由规则排列的支柱连接到内部基底层。经超声波处理和1%十二烷基硫酸钠(SDS)处理后，角质层可基本完整地分离出来，不含任何细胞物质。纯化的角质层在基础角质层中呈负性物质。角质层在巯基还原剂中的溶解度不同，对各种蛋白水解酶和氨基酸组成的敏感性不同。支柱、基底层和内部皮质层由胶原蛋白组成，胶原蛋白通过二硫键广泛交联。外皮质层似乎主要含有非胶原蛋白，这些蛋白通过不可还原的共价键进行广泛的交联。用还原剂从角质层中提取的胶原蛋白可以通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离成表观分子量不同的八个主要物种。
The adult cuticle of the soil nematode, Caenorhabditis elegans, is a proteinaceous extracellular structure elaborated by the underlying layer of hypodermal cells during the final molt in the animal's life cycle. The cuticle is composed of an outer cortical layer connected by regularly arranged struts to an inner basal layer. The cuticle can be isolated largely intact and free of all cellular material by sonication and treatment with 1% sodium dodecyl sulfate (SDS). Purified cuticles exhibit a negative material in the basal cuticle layer. The cuticle layers differ in their solubility in sulfhydryl reducing agents, susceptibility to various proteolytic enzymes and amino acid composition. The struts, basal layer, and internal cortical layer are composed of collagen proteins that are extensively cross-linked by disulfide bonds. The external cortical layer appears to contain primarily noncollagen proteins that are extensively cross-linked by nonreducible covalent bonds. The collagen proteins extracted from the cuticle with a reducing agent can be separated by SDS-polyacrylamide gel electrophoresis into eight major species differing in apparent molecular weight.