Expression patterns of β1-related α integrin subunits in murine lens during embryonic development and wound healing
Expression patterns of β1-related α integrin subunits in murine lens during embryonic development and wound healing
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DOI：
10.1080/02713680490513137
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发表时间：
2004-07-01
影响因子：
2
通讯作者：
Ohnishi, Y
中科院分区：
文献类型：
--
作者：
Barbour, W;Saika, S;Ohnishi, Y
目的.目的研究β 1相关α整合素亚单位在小鼠透镜上皮细胞中的表达模式，比较胚胎纤维分化和损伤诱导的上皮细胞间质转化（EMT）。成年小鼠C57 BL/6型，怀孕以及眼睛受伤，在不同的时间过程间隔处死。取胚胎眼和损伤眼石蜡切片，免疫组化染色检测整合素A亚基的表达。胚胎透镜上皮细胞主要表达α 3和α 5亚基，而胚胎纤维细胞表达α 2、α 5和α 6亚基。成年透镜上皮细胞表达α 3和α 6亚单位，而受损的透镜细胞表达α 2、α 3和α 6整合素亚单位。透镜上皮细胞在胚胎纤维分化和EMT过程中的表型变化的特征在于整合素亚基的不同表达，这是细胞外基质条件改变和每个过程中招募的细胞信号传导途径改变的结果。
Purpose. To study the expression patterns of beta1-related alpha integrin subunits in murine lens epithelial cells, comparing embryonic fiber differentiation with injury-induced epithelia] mesenchymal transition (EMT).Methods. Adult mice type C57BL/6, pregnant as well as with an eye injured, were sacrificed at different time-course intervals. The embryonic and the injured eyes were obtained and deparaffinized sections of these eyes were processed for immunohistochemistry staining for detection of integrin a subunits.Results. Embryonic lens epithelial cells expressed primarily alpha3 and alpha5 subunits, whereas embryonic fiber cells expressed alpha2, alpha5, and alpha6 subunits. Adult lens epithelial cells expressed alpha3, and alpha6 subunits, whereas injured lens cells expressed alpha2, alpha3, and alpha6 integrin subunits.Conclusions. The phenotypic changes of lens epithelial cells during embryonic fiber differentiation and EMT are characterized by different expression of integrin subunits as a result both of the altered extracellular matrix conditions and of the altered cell signaling pathways recruited in each process.