Visual detection of in vitro nucleic acid replication by submicro- and nano-sized materials
Visual detection of in vitro nucleic acid replication by submicro- and nano-sized materials
复制标题
亚微米和纳米材料体外核酸复制的视觉检测
DOI：
10.1016/j.bios.2020.112602
复制
发表时间：
2020
影响因子：
12.6
通讯作者：
Xiahong Xu
中科院分区：
文献类型：
--
作者：
Liu Wang;Kaiyu He;Omer Sadak;Xinquan Wang;Qiang Wang;Xiahong Xu
体外核酸复制的快速发展为临床诊断、食品安全检测和环境监测提供了有力的工具。各种等温核酸扩增方法的成功实施使得能够在没有热循环仪参与的情况下快速复制靶序列。需求点分析在用户友好、即时结果分析、低制造和消耗成本方面具有很大的优势。为了应对需求点分析的巨大挑战，开发简单快速的可视化方法变得至关重要。亚微米和纳米材料具有独特的表面性质，这使得它们能够对DNA扩增子做出快速反应。它们独特的光学、磁性、催化和其他物理/化学性质经常被用于体外核酸复制的视觉检测。在此，我们的目的是审查的亚微米和纳米材料为基础的视觉检测方法的核酸扩增。可视化方法根据设计策略（例如LSPR、桥连絮凝、发光、催化反应、分离等）进行分类。介绍了每种策略的基本原理、优缺点。讨论了其在核酸靶标和非核酸靶标分析中的应用。主要的挑战和未来的研究方向也突出在这个迅速崛起的领域。
The rapid growth ofin vitronucleic acid replication has offered a powerful tool for clinical diagnosis, food safety detection and environmental monitorning. Successful implementation of various isothermal nucleic acid amplification methods enables rapid replication of target sequences without the participant of a thermal cycler. Point-of-need analysis possesses great superiorities in user-friendly, instant results analysis, low manufacturing, and consumable costs. To meet the great challenge of point-of-need analysis, developing simple and rapid visual methods becomes crucial. Submicro- and nanomaterials possess unique surface properties, which enables their rapid response to DNA amplicons. Their unique optical, magnetic, catalytic, and other physical/chemical properties have been frequently employed for the visual detection ofin vitronucleic acid replications. Herein, we aim to review the submicro- and nanomaterials-based visual methods for detection of nucleic acid amplification. The visual methods are classified according to the designing strategies (e.g. LSPR, bridging flocculation, luminescence, catalytic reaction, separation, etc.). The basic principles, merits and drawbacks of each strategy are described. The application in analysis of nucleic acid targets and non-nucleic acid targets are discussed. The main challenges and future research directions are also highlighted in this rapidly emerging field.