Error rates in dental microwear quantification using scanning electron microscopy.

Error rates in dental microwear quantification using scanning electron microscopy.
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使用扫描电子显微镜量化牙科微磨损的错误率。

DOI:
10.1002/sca.4950270105
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发表时间:
2006
期刊:
影响因子:
--
通讯作者:
M. Teaford
M. Teaford
中科院分区:
工程技术4区
文献类型:
--
作者:
F. Grine;P. Ungar;M. Teaford

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在现存的灵长类动物中,饮食习惯和牙齿微磨损之间存在一定程度的相关性,这使得人们能够对史前饮食做出推断。已经使用了几种技术来量化微磨损,但每种技术得出的结果是否具有可比性还没有得到证明。此外,到目前为止,还没有记录微磨损量化的观察者内或观察者间的误差率。我们在这里使用Microware 4.0评估观察者内和观察者间的误差,并使用该领域中最广泛使用的三种方法来评估技术间的可比性。这项研究记录了观察者内部的整体错误率约为7%,观察者间的整体错误率约为9%。观察者内部和观察者间的误差似乎都受到被测量显微照片的性质的很大影响。在任何情况下,不同观察者使用MICROWARE 4.0获得的结果都没有显著差异,并且观察者之间关于任何给定参数的显微照片的排序存在合理程度的一致性。通过使用不同的定量技术获得的结果差异很大,总体技术间错误率约为19%。几个变量,包括放大系数的不同,扫描电子显微镜千伏设置的不同,以及标本-检测器关系的不同,无疑导致了三种方法之间的差异,但我们无法评估它们的相对重要性。微磨损量化允许广泛的饮食类别之间的差异,但在定义种群(或物种)之间的模式差异或记录一个分类群内季节性减轻的差异时,应考虑观察者内和观察者间误差的幅度。鉴于不同方法的使用带来的误差,我们建议当前的研究人员采用一致的技术,如微波软件包所提供的技术来建立通用的微磨料数据库。
There is a degree of correlation between dietary habits and dental microwear in extant primates, and this has enabled inferences to be made about prehistoric diets. Several techniques have been used to quantify microwear, but the comparability of results derived from each has not been demonstrated. Moreover, neither intra- nor interobserver error rates in microwear quantification have been documented to date. We here assess intra- and interobserver error using Microware 4.0, and evaluate intertechnique comparability using the three methods that have been most widely employed in the field. This study documents an overall intraobserver error rate of about 7%, and an overall interobserver error rate of some 9%. Both intra- and interobserver error appears to be influenced substantially by the nature of the micrograph being measured. In no instance did the results obtained by different observers using Microware 4.0 differ significantly, and there was a reasonable degree of interobserver consistency in the rank ordering of micrographs in relation to any given parameter. The results obtained through the use of different quantitative techniques differed significantly, with an overall intertechnique error rate of approximately 19%. Several variables, including differences in magnification factor, scanning electron microscope kV settings, and specimen-detector relationships undoubtedly contribute to the differences among the three methods, but we were not able to assess their relative importance. Microwear quantification permits distinctions between broad dietary categories, but the margin of intra- and interobserver error should be taken into account when defining pattern differences between populations (or species) or when documenting seasonally mitigated differences within a taxon. In view of the error introduced by the use of different methods, we suggest that a consistent technique, such as offered by the Microware software package, be adopted by current researchers to establish a common microwear database.
现代哺乳动物牙齿微磨损和饮食的综述。
DOI: --
发表时间: 1988
期刊: Scanning microscopy
影响因子: --
作者:
Teaford,MF
通讯作者: Teaford,MF