Bone morphogenic protein-2 (BMP-2) loaded nanoparticles mixed with human mesenchymal stem cell in fibrin hydrogel for bone tissue engineering
Bone morphogenic protein-2 (BMP-2) loaded nanoparticles mixed with human mesenchymal stem cell in fibrin hydrogel for bone tissue engineering
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DOI：
10.1016/j.jbiosc.2009.05.021
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Na, Kun
中科院分区：
文献类型：
--
作者：
Park, Keun-Hong;Kim, Hyemin;Na, Kun
最近的组织工程努力集中在使用天然或合成的基质，这种基质结合了可生物降解的特性和蛋白质输送载体的特性，允许植入细胞行为和增强组织再生。本研究的主要目的是评价三维纤维蛋白复合纳米载体骨形态发生蛋白-2(BMP-2)异位成骨的可行性，以诱导人骨髓间充质干细胞(HMSCs)向成骨方向分化。我们的评估结果表明，纳米颗粒中负载的生长因子的刺激显著地影响了植入纤维蛋白结构中的hMSCs的成骨分化。通过RT-PCR、实时定量聚合酶链式反应、Western blotting、组织学和免疫组织化学方法检测纤维蛋白构建物中hMSCs的成骨活性，观察到含有生长因子(BMP-2)纳米粒的纤维蛋白构建体在组织学上有明显的均质骨形成。通过上述检测技术，包裹在纤维蛋白结构中的BMP-2纳米颗粒与对照或不含纳米颗粒的BMP-2纤维蛋白结构相比，证明了hMSCs对骨再生的更有效的作用。在目前的研究中，我们得出结论，含有BMP-2纳米颗粒的纤维蛋白构建物将是一种有希望的方法，通过它可以促进骨再生。(C)2009年，日本生物技术学会。版权所有。
Recent tissue engineering efforts have been focused on the use of natural or synthetic matrices which combine the characteristics of biodegradable properties with those of protein delivery vehicles, allowing for implanted cell actions and enhanced tissue regeneration. The principal objective of this study was to assess the feasibility of ectopic bone formation in a three-dimensional fibrin construct mixed with bone morphogenic protein-2 (BMP-2) loaded in nano-carriers for the osteogenic differentiation of human mesenchymal stem cells (hMSCs). The results of our evaluation showed that the osteogenic differentiation of hMSCs embedded in the fibrin construct was affected significantly by the stimulation of growth factors loaded in nanoparticles. When the osteoinduction activity of hMSCs in fibrin construct was evaluated in an in vitro test followed by RT-PCR, real time-QPCR, Western blotting, histological and immunohistochemical examinations, significant homogeneous bone formation was observed histologically throughout the fibrin construct containing the growth factor (BMP-2) loaded into the nanoparticles. With the above detection techniques, the BMP-2-loaded nanoparticles encapsulated in fibrin constructs evidenced more potent effects of hMSCs on bone regeneration as compared to the control or BMP-2 loaded fibrin constructs without nanoparticles. In the current study, we conclude that fibrin constructs containing BMP-2 loaded nanoparticles will be a promising method by which bone regeneration can be enhanced. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.