A novel model of mild traumatic brain injury for juvenile rats.
A novel model of mild traumatic brain injury for juvenile rats.
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DOI：
10.3791/51820
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发表时间：
2014-12-08
期刊：
影响因子：
--
通讯作者：
Esser MJ
中科院分区：
文献类型：
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作者：
Mychasiuk R;Farran A;Angoa-Perez M;Briggs D;Kuhn D;Esser MJ
尽管越来越多的证据表明，儿童时期是运动相关脑震荡、机动车事故和跌倒导致轻度创伤性脑损伤(MTBI)的主要风险期，但以前还没有针对这一重要发育方面开发出可靠的mTBI动物模型。改进的减重技术采用了对自由移动的啮齿动物头部的掠过冲击，向大脑传递加速、减速和旋转力。当应用于幼年大鼠时，这种改进的减重技术产生了具有脑震荡后症状的临床相关行为结果。该技术是一种快速应用的程序，死亡率极低，使其成为治疗学高通量研究的理想选择。此外，由于该手术涉及闭合头部的轻微损伤，因此可以很容易地用于重复脑损伤的研究。由于这项技术的简单性和损伤病理生理学的临床相关生物力学，改进的重量下降技术为研究人员提供了一种可靠的mTBI模型，可用于广泛的行为、分子和遗传学研究。
Despite growing evidence that childhood represents a major risk period for mild traumatic brain injury (mTBI) from sports-related concussions, motor vehicle accidents, and falls, a reliable animal model of mTBI had previously not been developed for this important aspect of development. The modified weight-drop technique employs a glancing impact to the head of a freely moving rodent transmitting acceleration, deceleration, and rotational forces upon the brain. When applied to juvenile rats, this modified weight-drop technique induced clinically relevant behavioural outcomes that were representative of post-concussion symptomology. The technique is a rapidly applied procedure with an extremely low mortality rate, rendering it ideal for high-throughput studies of therapeutics. In addition, because the procedure involves a mild injury to a closed head, it can easily be used for studies of repetitive brain injury. Owing to the simplistic nature of this technique, and the clinically relevant biomechanics of the injury pathophysiology, the modified weight-drop technique provides researchers with a reliable model of mTBI that can be used in a wide variety of behavioural, molecular, and genetic studies.