Three-Dimensional Printing and Angiogenesis: Tailored Agarose-Type I Collagen Blends Comprise Three-Dimensional Printability and Angiogenesis Potential for Tissue-Engineered Substitutes
Three-Dimensional Printing and Angiogenesis: Tailored Agarose-Type I Collagen Blends Comprise Three-Dimensional Printability and Angiogenesis Potential for Tissue-Engineered Substitutes
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DOI：
10.1089/ten.tec.2017.0234
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Fischer, Horst
中科院分区：
文献类型：
--
作者：
Kreimendahl, Franziska;Koepf, Marius;Fischer, Horst
三维(3D)生物打印是一种很有前途的制造细胞负载的组织工程化结构的技术。然而，较大的组织替代品需要一个血管化的网络来确保营养供应。因此，需要结合3D打印和细胞诱导血管形成的定制生物墨水。我们假设，由琼脂糖-I型胶原和琼脂-纤维蛋白原制成的定制水凝胶混合物可以进行3D打印，并将允许人脐静脉内皮细胞和人真皮成纤维细胞形成毛细血管样结构。标本浇铸，孵育14天，进行免疫组织学和双光子激光扫描显微镜分析。使用按需打印系统检查了水凝胶混合物的3D打印性能。并对其流变性进行了研究。在浓度为0.2%或0.5%的胶原蛋白和0.5%琼脂糖的琼脂糖-I型胶原水凝胶共混物中观察到大量的毛细血管网络形成。此外，与相应的单一组分相比，琼脂糖胶原蛋白共混物的储存模数显著增加(分别为448pA、148pA和1551pA)。添加胶原和纤维蛋白原都不会显著影响打印分辨率。总之，我们提出了一种量身定做的水凝胶混合物，它可以3D打印，并平行显示细胞诱导血管形成的能力。
Three-dimensional (3D) bioprinting is a promising technology for manufacturing cell-laden tissue-engineered constructs. Larger tissue substitutes, however, require a vascularized network to ensure nutrition supply. Therefore, tailored bioinks combining 3D printability and cell-induced vascularization are needed. We hypothesize that tailored hydrogel blends made of agarose-type I collagen and agarose-fibrinogen are 3D printable and will allow the formation of capillary-like structures by human umbilical vein endothelial cells and human dermal fibroblasts. Samples were casted, incubated for 14 days, and analyzed by immunohistology and two-photon laser scanning microscopy. The 3D printability of the hydrogel blends was examined using a drop-on-demand printing system. The rheological behavior was also investigated. Substantial capillary network formation was observed in agarose-type I collagen hydrogel blends with concentrations of 0.2% or 0.5% collagen and 0.5% agarose. Furthermore, storage moduli of agarose-collagen blends were significantly increased compared to those of the corresponding single components (448 Pa for 0.5% agarose, 148 Pa for 0.5% collagen, and 1551 Pa for 0.5% agarose-0.5% collagen). Neither the addition of collagen nor fibrinogen significantly impaired the printing resolution. In conclusion, we present a tailored hydrogel blend that can be printed in 3D and in parallel exhibits cell-induced vascularization capability.