SPECIFIC BINDING OF A BASIC PEPTIDE FROM HIV-1 REV
SPECIFIC BINDING OF A BASIC PEPTIDE FROM HIV-1 REV
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DOI：
10.1002/j.1460-2075.1992.tb05152.x
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发表时间：
1992-03-01
期刊：
影响因子：
11.4
通讯作者：
SHARP, PA
中科院分区：
文献类型：
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作者：
KJEMS, J;CALNAN, BJ;SHARP, PA
人类免疫缺陷病毒 I 型 (HIV-1) 编码一种调节蛋白 Rev，它是不完全剪接的病毒 mRNA 的细胞质表达所必需的。 Rev 活性是通过与位于 HIV-1 env 区域内的顺式作用 Rev 反应元件 (RRE) 特异性结合来介导的。通过RNA足迹、修饰干扰实验和突变分析研究了对应于RRE (IIB RNA)的37个核苷酸的单体Rev结合位点。令人惊讶的是，对应于 Rev 基本结构域的 17 个氨基酸肽，在基本相同的核苷酸处特异性结合到该位点，并可能诱导额外的碱基配对。 Rev蛋白和相关肽主要与位于单链和双链区域连接处以及位于螺旋内的附加位点的两组核苷酸相互作用。这表明负责特定 RNA 结合的蛋白质结构域可能非常小，并且 RNA 和蛋白质之间的相互作用可能会诱导两种成分的结构。
Human immunodeficiency virus type I (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA. Rev activity is mediated through specific binding to a cis-acting Rev responsive element (RRE) located within the env region of HIV-1. A monomer Rev binding site corresponding to 37 nucleotides of the RRE (IIB RNA) was studied by RNA footprinting, modification interference experiments and mutational analysis. Surprisingly, a 17 amino acid peptide, corresponding to the basic domain of Rev, binds specifically to this site at essentially identical nucleotides and probably induces additional base pairing. The Rev protein and related peptide interact primarily with two sets of nucleotides located at the junction of single and double stranded regions, and at an additional site located within a helix. This suggests that the domains of proteins responsible for specific RNA binding can be remarkably small and that the interaction between RNA and protein can probably induce structure in both constituents.