Determining the self-incompatibility alleles of Japanese apple cultivars
Determining the self-incompatibility alleles of Japanese apple cultivars
复制标题
确定日本苹果品种的自交不亲和等位基因
DOI：
10.21273/hortsci.32.7.1258
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发表时间：
1997
期刊：
影响因子：
1.9
通讯作者：
N. Weeden
中科院分区：
文献类型：
--
作者：
K. Sakurai;S. Brown;N. Weeden
采用Janssens等人开发的等位基因特异性聚合酶链式反应扩增和限制性内切酶消化系统测定了15个日本苹果品种的S等位基因。 （1995）。在八个二倍体品种中鉴定出两个 S 等位基因，在三个三倍体品种中鉴定出两个 S 等位基因，在其余四个二倍体品种中鉴定出一个 S 等位基因。两个品种的 S 等位基因与其亲缘预测的不同，在对品种与其运动进行比较时，发现了身份问题。该技术有助于表明亲本在三倍体中贡献了未减少的配子。
The S alleles of 15 Japanese apple cultivars were determined by using the allele-specific polymerase chain reaction amplification and restriction enzyme digestion system developed by Janssens et al. (1995). Both S alleles were identified in eight diploid cultivars, two S alleles in three triploid cultivars, and one S allele in the remaining four diploid cultivars. Two cultivars had S alleles different than those predicted by their parentage, and in one comparison of a cultivar with its sport, an identity problem was discovered. The technique helped to indicate the parent contributing the unreduced gamete in triploids.