CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN RAT FOREBRAIN - AUTORADIOGRAPHIC IDENTIFICATION
CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN RAT FOREBRAIN - AUTORADIOGRAPHIC IDENTIFICATION
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DOI：
10.1126/science.6328656
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
KUHAR, MJ
中科院分区：
文献类型：
--
作者：
DESOUZA, EB;PERRIN, MH;KUHAR, MJ
用碘-125标记的绵羊促肾上腺皮质激素释放因子（CRF）类似物在氨基酸残基21和32处分别被正亮氨酸和酪氨酸取代，通过放射自显影鉴定大鼠前脑中的CRF受体。CRF的高亲和力受体被发现在大鼠前脑的离散区域中，包括新皮质的I层和IV层、正中隆起的外层、杏仁核的外侧核和纹状体。这些结果与早期CRF免疫组化分布的研究结果一致，表明内源性CRF在调节中枢神经系统活动中具有生理作用。
Corticotropin-releasing factor (CRF) receptors were identified in rat forebrain by autoradiography with an iodine-125-labeled analog of ovine CRF substituted with norleucine and tyrosine at amino acid residues 21 and 32, respectively. High-affinity receptors for CRF were found in discrete areas of rat forebrain, including laminae I and IV of the neocortex, the external layer of the median eminence, the lateral nucleus of the amygdala, and the striatum. These results are consistent with earlier findings on the immunohistochemical distribution of CRF and suggest that endogenous CRF has a physiological role in regulating activity of the central nervous system.