Air-plasma treatment promotes bone-like nano-hydroxylapatite formation on protein films for enhanced in vivo osteogenesis
Air-plasma treatment promotes bone-like nano-hydroxylapatite formation on protein films for enhanced in vivo osteogenesis
复制标题
空气等离子体处理促进蛋白膜上骨样纳米羟基磷灰石的形成，从而增强体内成骨作用
DOI：
10.1039/c9bm00020h
复制
发表时间：
2019
影响因子：
6.6
通讯作者：
Chuanbin Mao
中科院分区：
文献类型：
--
作者：
Qing Zhang;Lu Ma;Shengnan Zheng;Yaru Wang;Meilin Feng;Yajun Shuai;Bo Duan;Xin Fan;Mingying Yang;Chuanbin Mao
将羟基磷灰石（HAp）引入生物大分子材料是提高其骨再生能力的有效途径。因此，需要开发一种简便的方法来实现这一目标。在这里，我们表明，一个简单的空气等离子体处理的丝素蛋白（SF）薄膜5分钟诱导骨板状纳米HAp（nHAp）在其表面上的形成和所得材料有效地增强体内成骨。空气等离子体处理的SF膜（A-SF）与原始SF膜（SF）相比呈现出表面纳米柱和增强的亲水性，使得A-SF和SF膜分别诱导形成板状/多晶和针状/少晶nHAp。将矿化的A-SF和SF膜（分别称为A-SF-nHAp和SF-nHAp）及其非矿化的对应物与大鼠间充质干细胞接种并皮下植入大鼠模型中。A-SF-nHAp和A-SF膜由于其独特的纳米形貌而在4周内表现出比SF-nHAp和SF膜更有效的骨形成，其中A-SF-nHAp膜比A-SF膜更有效。这项工作表明，空气等离子体处理和随后的nHAp矿化的组合最有效地促进骨形成。我们的基于等离子体的方法是一种有吸引力的方法，以提高基于蛋白质的生物材料的骨再生能力。
Introducing hydroxylapatite (HAp) into biomolecular materials is a promising approach to improve their bone regenerative capability. Thus a facile method needs to be developed to achieve this goal. Here we show that a simple air-plasma treatment of silk fibroin (SF) films for 5 min induced the formation of bone-like plate-shaped nano-HAp (nHAp) on their surface and the resultant material efficiently enhanced in vivo osteogenesis. The air-plasma-treated SF films (termed A-SF) presented surface nano-pillars and enhanced hydrophilicity compared to the pristine SF films (termed SF), making the A-SF and SF films induce the formation of plate-shaped/more-crystalline and needle-like/less-crystalline nHAp, respectively. The mineralized A-SF and SF films (termed A-SF-nHAp and SF-nHAp, respectively) and their non-mineralized counterparts were seeded with rat mesenchymal stem cells and subcutaneously implanted into the rat models. The A-SF-nHAp and A-SF films exhibited more efficient bone formation than the SF-nHAp and SF films in 4 weeks due to their unique nanotopography, with the A-SF-nHAp films being more efficient than the A-SF films. This work shows that a combination of the air-plasma treatment and the subsequent nHAp mineralization most efficiently promotes bone formation. Our plasma-based method is an attractive approach to enhance the bone regenerative capacity of protein-based biomaterials.