Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients
Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients
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DOI：
10.1128/jcm.00738-14
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发表时间：
2014-08-01
影响因子：
9.4
通讯作者：
Ferguson, David
中科院分区：
文献类型：
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作者：
Landry, Marie L.;Ferguson, David
为了将 Simplexa Flu A/B 和 RSV PCR 与细胞离心涂片免疫荧光和实验室开发的 TaqMan PCR 方法进行比较，对 445 个鼻咽样本进行了测试。其中，199 例呈阳性（46 例呼吸道合胞病毒 [RSV]、120 例甲型流感、33 例乙型流感），246 例呈阴性。直接荧光抗体测定 (DFA) 检测到 132 个 (66.3%) 阳性样本，Simplexa 直接检测到 162 个 (81.4%)，使用提取物的 Simplexa 检测到 177 个 (88.9%)，实验室开发的 TaqMan PCR 参考方法检测到 199 个 (100%)。特异性分别为 99.6%、100%、100% 和 100%。两种 Simplexa 方法比 DFA 更灵敏 (P = 0.0001)，但不如 TaqMan 逆转录酶 PCR (P = 0.0001) 灵敏。
To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-antibody assay (DFA) detected 132 (66.3%) positive samples, Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%). The specificities were 99.6%, 100%, 100%, and 100%, respectively. The two Simplexa methods were more sensitive than the DFA (P = 0.0001) but less sensitive than the TaqMan reverse transcriptase PCR (P = 0.0001).