Impact of serial thermotherapy on sugarcane mosaic virus titre and regeneration in sugarcane.
Impact of serial thermotherapy on sugarcane mosaic virus titre and regeneration in sugarcane.
复制标题
DOI：
10.1080/03235400310001595630
复制
发表时间：
2003-01-01
影响因子：
1
通讯作者：
Doraisamy, S.
中科院分区：
文献类型：
--
作者：
Balamuralikrishnan, M.;Sabitha Doraisamy, Sabitha Doraisamy;Doraisamy, S.
以两个甘蔗品种（Co740和CoC671）为试材，采用不同处理系列的热水热处理，研究其对甘蔗花叶病毒（SCMV）增殖的影响。在种植经处理的茎段后的不同时间间隔记录对病毒滴度和茎段再生的观察。采用直接抗原包被ELISA法，制备了抗SCMV地方株的多克隆抗血清。所有热处理均影响两个品种的茎段再生。虽然没有一个处理系列产生无病毒的植物，SCMV滴度是相对减少的再生植物在早期阶段（种植后4周），在严重的热水处理系列，但在sett发芽急剧减少观察。然而，在所有处理系列中，SCMV滴度在稍后（种植后8周）回升。SCMV滴度的早期降低可用于在分生组织培养中获得更多的脱毒植株。
Serial thermotherapy with hot water involving different treatment series was followed in two sugarcane cultivars (Co 740 and CoC 671) for assessing its effect on sugarcane mosaic virus (SCMV) titre in the regenerated plants. Observations on virus titre and sett regeneration were recorded at different intervals after planting the treated setts. The direct antigen coating ELISA method was followed up with the polyclonal antisera raised against the widespread local SCMV strain. All the thermo treatments affected the sett regeneration in both cultivars. Although none of the treatment series produced virus-free plants, the SCMV titre was comparatively reduced in the regenerated plants at an early phase (4 weeks after planting) in severe hot water treatment series, but drastic reduction in sett germination was observed. However, the SCMV titre picked up later (8 weeks after planting) in all the treatment series. The early reduction in SCMV titre could be utilized for obtaining more virus-free plants in meristem culture.