Muscle-based gene therapy and tissue engineering to improve bone healing
Muscle-based gene therapy and tissue engineering to improve bone healing
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DOI：
10.1097/01.blo.0000031984.92980.5d
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发表时间：
2002-10-01
影响因子：
4.2
通讯作者：
Huard, J
中科院分区：
文献类型：
--
作者：
Young, BH;Peng, HR;Huard, J
骨折愈合失败是骨科的一个重要问题，常见于舟骨骨折、高能量损伤和骨质疏松症患者。目前的治疗方法往往导致不良的结果和供体部位的发病率。基因治疗一直是最近许多促进骨骼愈合的研究的重点。在目前的综述中，作者特别评价了肌肉来源的细胞作为基因载体和可诱导的成骨细胞来促进骨再生的应用。肌肉来源的细胞已经被用来运送骨形态发生蛋白-2和产生异位骨。这些细胞表达骨钙素，在新生骨中发现，通常由成骨细胞和骨细胞占据。最后，研究表明，肌源性细胞结合体外基因治疗可以修复临界大小的颅骨缺损。
Failed fracture healing is a significant problem in orthopaedics, often seen in patients with scaphoid fractures, high-energy injuries, and osteoporosis. Current treatments often result in poor outcomes and donor site morbidity. Gene therapy has been the focus of much recent research to improve bone healing. In the current review, the authors specifically evaluate the use of muscle-derived cells as a gene delivery vehicle and inducible osteoprogenitor cell that can enhance bone regeneration. Muscle-derived cells have been used to deliver bone morphogenetic protein-2 and produce ectopic bone. These cells express osteocalcin and have been found within newly generated bone in locations normally occupied by osteo-blasts and osteocytes. Finally, it is shown that muscle-derived cells coupled with ex vivo gene therapy can heal critical-sized calvarial defects.