Delineation of the bacterial composition in exogenous endophthalmitis using 16S rDNA sequencing
Delineation of the bacterial composition in exogenous endophthalmitis using 16S rDNA sequencing
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DOI：
10.1007/s10792-022-02428-w
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发表时间：
2022-07
影响因子：
1.6
通讯作者：
Benxiang Qi;Bining Zhang;Baoxia Yang;Huabo Chen;Zhichao Ren;Xiubin Ma;Junling Jing;T. Xia
中科院分区：
文献类型：
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作者：
Benxiang Qi;Bining Zhang;Baoxia Yang;Huabo Chen;Zhichao Ren;Xiubin Ma;Junling Jing;T. Xia
目的应用16S rDNA测序方法检测外源性眼内炎的细菌谱，包括外伤后、白内障手术后、滤过泡相关和玻璃体内治疗相关的眼内炎。对这些样品进行常规培养和16S rDNA测序。结果24例标本中有11例(45.8%)细菌培养阳性，且每份标本只检出一种细菌。在相应的测序结果中，11个培养阳性菌都能被鉴定出来，但只有4个菌株是测序中排名第一的病原菌。16S rDNA测序共分离出567株细菌，前五位分别是假单胞菌、表皮葡萄球菌、葡萄球菌、链球菌和粪肠球菌。不同类型的眼内炎的优势菌株不同，但总体细菌谱没有明显差异。细菌图谱包括假单胞菌、链球菌、葡萄球菌、放线菌_未分类、热杆菌和日杆菌。房水细菌图谱与环境污染细菌有较高的重叠性。结论16S rDNA测序在阳性检测率和细菌鉴定数量方面均优于传统的培养方法。但用16S rDNA测序法诊断眼内炎存在环境污染的风险。不同类型的眼内炎表现出不同的细菌组成。
PurposeTo determine the bacterial spectrum of exogenous endophthalmitis of different origins, namely, posttraumatic, postcataract surgery, filtering bleb-associated, and intravitreal treatment-related endophthalmitis, using the 16S rDNA sequencing method.MethodsAqueous humor or vitreous humor samples were collected from 24 endophthalmitis patients. Traditional cultivation and 16S rDNA sequencing were conducted with these samples. Three senile cataract controls and one intraocular irrigating solution were used as sequencing control.ResultsEleven of the 24 samples (45.8%) obtained positive bacterial cultivation, and each sample positive for only one species. The 11 culture-positive species could all be identified in their corresponding sequencing results, but only four strains being the top one pathogen in the sequencing. A total of 567 species were isolated using 16S rDNA sequencing, with the top five species beingPseudomonassp.,Staphylococcus epidermidis,Staphylococcussp.,Streptococcussp., andEnterococcus faecalis. The dominant bacterial strains varied among the different endophthalmitis categories but with no significant difference in the overall bacterial spectrum. Bacterial atlas containingPseudomonas,Streptococcus,Staphylococcus,Actinomycetales_unclassified,Thermus, andJanibacterwas shared by the four categories. Aqueous humor bacterial profile showed a higher overlap with contaminating bacteria from the environment.Conclusions16S rDNA sequencing is more efficient for endophthalmitis pathogen screening than the traditional cultivation method in terms of positive detection rate and the number of bacteria identified. But the risk of environmental contamination exists when using 16S rDNA sequencing method for endophthalmitis diagnosis. Different categories of endophthalmitis displayed diversified bacterial composition.