Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags
Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags
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DOI：
10.1111/j.1471-8286.2005.01056.x
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发表时间：
2005-12-01
期刊：
影响因子：
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通讯作者：
Davidson, WS
中科院分区：
文献类型：
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作者：
Ng, SHS;Chang, A;Davidson, WS
表达序列标签(EST)中简单序列重复序列(SSR)的检测将潜在的微卫星标记与特定基因连接起来，产生I型标记。使用电子计算机方法，我们从大西洋鲑鱼基因组研究项目EST数据库中识别了1975个SSR。我们设计了扩增158个SSR的引物，其中65个扩增出76个座位(包括11个重复的座位)。在来自7个种群的24条大西洋鲑鱼中，76个座位中有61个是变异的，其中96%的标记还扩增了其他鲑鱼的DNA。其中16个与SSR相关的EST的功能已经确定，证实它们是I型标记。
The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty-one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers.