ANTIGENS OF THE ERYTHROCYTIC STAGES OF THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM DETECTED BY MONOCLONAL-ANTIBODIES
ANTIGENS OF THE ERYTHROCYTIC STAGES OF THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM DETECTED BY MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0166-6851(83)90025-7
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发表时间：
1983-01-01
影响因子：
1.5
通讯作者：
SCAIFE, J
中科院分区：
文献类型：
--
作者：
HALL, R;MCBRIDE, J;SCAIFE, J
小鼠抗P。用间接免疫荧光法和免疫沉淀法对恶性疟原虫单抗进行鉴定。在此基础上，确定了5组抗体和6类抗原。第一组抗体对包括配子体在内的所有血液阶段进行明亮、均匀、普遍的染色。其中三种抗体沉淀出一个35 kDa的代谢性标记分子(S)。其中一种沉淀50 kDa的抗原。第2组抗体在裂殖子中产生强烈的局部免疫荧光，在早期阶段产生微弱的扩散模式，沉淀出160kDA的生物合成标记分子。第3组抗体对所有无性阶段均有反应。对于裂殖子，无论是固定的还是非固定的制备方法，它们都会在周围产生强烈的染色。它们沉淀190 kDa的生物合成分子。第4组抗体与第3组抗体相同，只是它们仅限于裂殖体和裂殖子。它们还沉淀190 kDa的抗原。这些标记很容易被125I-乳过氧化物酶标记获得。一种抗体还会沉淀一组较小的多肽。V组抗体对含有色素的寄生虫以及红细胞中的包涵体产生非常明亮的模糊染色。它们沉淀出一系列160、60和35 kDa的分子，这些分子很容易被125I接触到。160 kDa的分子还被[35S]蛋氨酸标记。在开发疟疾疫苗和免疫诊断测试的背景下讨论了结果。
A range of 22 mouse anti-P. falciparum monoclonal antibodies was characterized by indirect immunofluorescence and immunoprecipitation. On the basis of these studies, 5 groups of antibodies and 6 classes of antigen were defined. Group 1 antibodies give bright, uniform, generalized staining of all blood stages including gametocytes. Three of these antibodies precipitate a metabolically labeled molecule(s) of 35 kDa [kilodalton]. One precipitates a 50 kDa antigen. Group 2 antibodies, which give strong localized immunofluorescence in merozoites, and a weak diffuse pattern in earlier stages, precipitate biosynthetically labeled molecules of 160 kDA. Group 3 antibodies react with all asexual stages. With merozoites they produce intense staining around the perimeter, both in fixed and unfixed preparations. They precipitate biosynthetic molecules of 190 kDa. Group 4 antibodies are identical to Group 3 except they are stage-restricted to schizonts and merozoites. They also precipitate 190 kDa antigens. These are readily accessible to 125I-lactoperoxidase labeling. One antibody also precipitates a set of smaller peptides. Group V antibodies produce very bright ill-defined staining of pigment-containing parasites, as well as of inclusions in the red cell. They precipitate a series of molecules of 160, 60 and 35 kDa which are readily accessible to 125I. The 160 kDa molecule is also labeled by [35S]methionine. Results are discussed in the context of the development of a malaria vaccine and immunodiagnostic tests.