Proliferation of astrocytes in vitro in response to cytokines. A primary role for tumor necrosis factor.
Proliferation of astrocytes in vitro in response to cytokines. A primary role for tumor necrosis factor.
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DOI：
10.4049/jimmunol.144.1.129
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发表时间：
1990-01
影响因子：
4.4
通讯作者：
K. Selmaj;M. Farooq;W. T. Norton;C. Raine;C. Brosnan
中科院分区：
文献类型：
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作者：
K. Selmaj;M. Farooq;W. T. Norton;C. Raine;C. Brosnan
在含血清培养基和化学成分确定的培养基中测定细胞因子对从成熟牛脑培养的星形胶质细胞的影响。结果表明，在无血清培养基中，人 TNF 以及较小程度的 IL-6 和淋巴毒素对星形胶质细胞具有促有丝分裂作用。 [3H]胸苷摄取增加可在体外36小时内检测到，并通过放射自显影证实其在星形胶质细胞中的存在。相比之下，IL-1α和IL-1β在无血清培养基中均不诱导星形胶质细胞增殖，但在72小时后与血清表现出一定的协同作用。通过细胞计数证实TNF和IL-6的增殖作用。根据 51Cr 释放量测定，所测试的细胞因子对星形胶质细胞均无毒性。没有检测到从同一来源纯化的少突神经胶质细胞的有丝分裂作用。研究结果支持活化炎症细胞产物在星形胶质细胞增殖发展中的作用，星形胶质细胞增殖可能导致中枢神经系统白质疾病（如多发性硬化症）中发现的反应性神经胶质增生。
The effect of cytokines on astrocytes cultured from mature bovine brain was determined both in a serum-containing medium and in a chemically-defined medium. The results showed that in serum-free medium, human TNF and, to a lesser degree, IL-6 and lymphotoxin, were mitogenic for astrocytes. Increased uptake of [3H]thymidine could be detected within 36 h in vitro and its presence in astrocytes was confirmed by autoradiography. In contrast, neither IL-1 alpha nor IL-1 beta induced astrocyte proliferation in serum-free medium but showed some synergistic effect with serum after 72 h. The proliferative effect of TNF and IL-6 was confirmed by cell counting. None of the cytokines tested was toxic for astrocytes as measured by 51Cr release. No mitogenic effect for oligodendroglia, purified from the same source, was detected. The results support a role for products of activated inflammatory cells in the development of astrocyte proliferation that may contribute to the reactive gliosis found in white matter diseases of the central nervous system such as multiple sclerosis.