Quantifying enamel demineralization from teeth with orthodontic brackets - a comparison of two methods. Part 1: repeatability and agreement
Quantifying enamel demineralization from teeth with orthodontic brackets - a comparison of two methods. Part 1: repeatability and agreement
复制标题
DOI：
10.1093/ejo/25.2.149
复制
发表时间：
2003-04-01
影响因子：
2.6
通讯作者：
Higham, SM
中科院分区：
文献类型：
--
作者：
Benson, PE;Pender, N;Higham, SM
本研究的目的是比较使用两种技术测量正畸托槽周围釉质脱矿的可重复性：数字转换的摄影载玻片和定量光诱导荧光（QLF）的计算机图像分析。15颗人类臼齿被切成两半，看起来像门牙。对牙齿进行单独编号，并将正畸托槽粘合到颊面。牙冠覆盖有耐酸清漆，除了与托槽的牙龈、咬合、近中和远侧边缘相邻的约1.5x3 mm的窗口。将窗户不同地暴露于去矿化凝胶0、3、7或14天，并除去耐酸清漆。拍摄牙齿的标准化摄影载玻片和QLF图像。这些在1周后重复。将载玻片转换为灰度数字格式，并使用Image-Pro Plus 3.0进行分析。使用定制软件存储、处理和分析QLF图像。将所有图像重新编码用于盲分析。检查了托槽的四个表面，仅分析了疑似脱矿的区域。两种技术的脱矿面积重复读数之间的一致性界限和平均差异相似（照片为-0.04+/-0.43，QLF为-0.10+/-0.63）。该区域的平均灰度（照片）和平均荧光损失（Δ F）（QLF）显示了可接受的一致性限度。对于来自QLF的面积测量，类内相关性（ICC）低于0.81，表明需要减少随机误差。有证据表明，重复读取照片的灰度值存在系统性偏倚（P
The aim of this investigation was to compare the repeatability of measuring enamel demineralization surrounding an orthodontic bracket using two techniques: computerized image analysis from digitally converted photographic slides and quantitative light-induced fluorescence (QLF). Fifteen human molars were halved and shaped to look like incisors. The teeth were individually numbered and orthodontic brackets bonded to the buccal surface. The crowns were covered with acid resistant varnish, except for windows approximately 1.5x3 mm adjacent to the gingival, occlusal, mesial, and distal edges of the bracket. The windows were variously exposed to a demineralizing gel for 0, 3, 7, or 14 days, and the acid resistant varnish was removed. Standardized photographic slides and QLF images of the teeth were taken. These were repeated after 1 week. The slides were converted to grey scale digital format and analysed using Image-Pro Plus 3.0. The QLF images were stored, processed, and analysed using customized software. All images were recoded for blind analysis. The four surfaces of the bracket were inspected and only areas of suspected demineralization were analysed. This was repeated after 1 week.The limits of agreement and mean difference between repeat readings of the area of demineralization were similar for both techniques (-0.04+/-0.43 for photographs and -0.10+/-0.63 for QLF). Mean grey level (photographs) and mean loss of fluorescence from that area (DeltaF) (QLF) showed acceptable limits of agreement. The Intra Class Correlation (ICC) was below 0.81 for the measurement of area from QLF, suggesting that random error needs to be reduced. There was evidence of systematic bias for the repeat readings of the grey levels from the photographs (P