Assessing Additivity of Cytotoxicity Associated with Disinfection Byproducts in Potable Reuse and Conventional Drinking Waters
Assessing Additivity of Cytotoxicity Associated with Disinfection Byproducts in Potable Reuse and Conventional Drinking Waters
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DOI：
10.1021/acs.est.0c00958
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发表时间：
2020-05-05
影响因子：
11.4
通讯作者：
Mitch, William A.
中科院分区：
文献类型：
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作者：
Lau, Stephanie S.;Wei, Xiao;Mitch, William A.
最近的研究使用单个消毒副产物（DBP）的测量浓度之和，通过其中国仓鼠卵巢（CHO）细胞毒性LC 50值加权，以估计消毒沃茨的DBP相关细胞毒性。这种方法假设细胞毒性是相加的，而不是协同或拮抗的。在这项研究中，我们评估了这一假设是否是有效的混合物含有消毒副产物的浓度比在真实的消毒沃茨。我们研究了CHO细胞的细胞毒性的定义DBP混合物的基础上，在8个饮用水和饮用水回用沃茨中测量的43个受管制和不受管制的DBP的浓度。使用三种实验方法支持加和性的假设。首先，我们证明了计算的添加剂毒性（CAT）和基于生物测定的DBP混合物计算的添加剂毒性（BCAT）在中位数基础上在12%以内。我们还发现了一个加性毒性反应（CAT近似BCAT）之间的监管和未监管的DBP类。最后，DBP子集混合物的经验生物细胞毒性，独立于计算的毒性，是累加的。这些结果支持使用细胞毒性效力加权DBP浓度的总和作为与真实的消毒沃茨中已知DBP相关的CHO细胞毒性的估计值的有效性。
Recent studies used the sum of the measured concentrations of individual disinfection byproducts (DBPs) weighted by their Chinese hamster ovary (CHO) cell cytotoxicity LC50 values to estimate the DBP-associated cytotoxicity of disinfected waters. This approach assumed that cytotoxicity was additive rather than synergistic or antagonistic. In this study, we evaluated whether this assumption was valid for mixtures containing DBPs at the concentration ratios measured in authentic disinfected waters. We examined the CHO cell cytotoxicity of defined DBP mixtures based on the concentrations of 43 regulated and unregulated DBPs measured in eight drinking and potable reuse waters. The hypothesis for additivity was supported using three experimental approaches. First, we demonstrated that the calculated additive toxicity (CAT) and bioassay-based calculated additive toxicity (BCAT) of the DBP mixtures agree within 12% on a median basis. We also found an additive toxicity response (CAT approximate to BCAT) between the regulated and unregulated DBP classes. Finally, the empirical biological cytotoxicity of the DBP subset mixtures, independent of the calculated toxicity, was additive. These results support the validity of using the sum of cytotoxic potency-weighted DBP concentrations as an estimate of the CHO cell cytotoxicity associated with known DBPs in real disinfected waters.