Prion diseases: infectious and lethal doses following oral challenge
Prion diseases: infectious and lethal doses following oral challenge
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DOI：
10.1099/vir.0.19037-0
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发表时间：
2003-07-01
影响因子：
3.8
通讯作者：
Riemer, C
中科院分区：
文献类型：
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作者：
Baier, M;Norley, S;Riemer, C
从感染了263K瘙痒病菌株的绝症仓鼠身上制备的脑匀浆被连续稀释并口服给几组仓鼠。未稀释的脑匀浆在所有接种的动物中都导致了临床瘙痒。随着匀浆稀释度的降低，感染率下降，免疫印迹法检测到感染后520天的健康幸存者中存在亚临床感染。用死于疾病的动物数量和免疫印迹阳性幸存者和患病仓鼠的组合数量来计算接种剂的LD50和ID50。该模型系统近似于TSE的传播，如新变种克雅氏病(VCJD)通过饮食暴露于感染剂，并表明，由于计算的LD50和ID50之间的差异相当小，临床病例数量不会因疾病的亚临床携带者数量而大大超过。
A brain homogenate prepared from a terminally ill hamster infected with scrapie strain 263K was serially diluted and administered orally to groups of hamsters. The undiluted brain homogenate led to clinical scrapie in all animals inoculated. The attack rate decreased with dilutions of the homogenate, and subclinical infections were identified among the healthy survivors at 520 days post-infection by Western blotting. The number of animals succumbing to disease and the combined number of Western blot-positive survivors plus diseased hamsters were used to calculate the LD50 and ID50 of the inoculum. The model system represents an approximation to the transmission of TSEs such as new variant Creutzfeldt-Jakob disease (vCJD) via dietary exposure to the infectious agent and suggests that, due to the rather small difference between the calculated LD50 and ID50, the number of clinical cases will not be vastly exceeded by the number of subclinical carriers of the disease.