Antibodies to the epidermal growth factor receptor block the biological activities of sarcoma growth factor.
Antibodies to the epidermal growth factor receptor block the biological activities of sarcoma growth factor.
复制标题
表皮生长因子受体抗体可阻断肉瘤生长因子的生物活性。
DOI：
10.1073/pnas.80.18.5627
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
DeLarco,JE
中科院分区：
文献类型：
--
作者：
Carpenter,G;Stoscheck,CM;Preston,YA;DeLarco,JE
用特异性抗表皮生长因子受体抗体评价了表皮生长因子受体系统在介导肉瘤生长因子生物学活性中的作用。有两类抗EGF受体抗体，一类是阻断125I标记的EGF(125I-EGF)的结合，另一类是不阻断结合但确实与完整细胞表面的部分EGF受体相互作用的抗体。这两种类型的抗血清都已被检测其影响SGF生物学活性的能力。阻断125I-EGF与其受体结合的抗血清可阻断EGF或SGF对人成纤维细胞DNA合成的诱导，但不能阻断其他多肽有丝分裂原的诱导。抗EGF受体抗血清的滴定表明存在一组抗体，可阻断EGF和SGF的作用部位。阻断~(125)I-EGF结合的EGF受体抗血清也能抑制正常细胞在软琼脂中依赖于SGF的贴壁非依赖性生长。不阻断~(125)I-EGF结合或EGF活性的EGF受体抗血清不抑制SGF的任何生物学活性。这些结果表明，SGF的有丝分裂活性和集落形成活性都需要EGF受体的占据。
The role of the epidermal growth factor (EGF) receptor system in mediating the biological activities of sarcoma growth factor (SGF) has been assessed by using specific anti-EGF receptor antibodies. There are two classes of anti-EGF receptor antibodies, those that block binding of 125I-labeled EGF (125I-EGF) and those that do not block binding but do interact with a portion of the EGF receptor on the surface of intact cells. Antisera of both types have been assayed for their capacity to affect the biological activities of SGF. The antisera that block 125I-EGF binding to its receptor block the induction of DNA synthesis in human fibroblasts by either EGF or SGF but not by other polypeptide mitogens. Titration of the anti-EGF receptor antiserum indicates the presence of one population of antibody that blocks the site of both EGF and SGF action. Antisera to the EGF receptor that block 125I-EGF binding also inhibited the SGF-dependent anchorage-independent growth of normal cells in soft agar. The antisera to the EGF receptor that does not block 125I-EGF binding or EGF activity did not inhibit any of the biological activities of SGF. The results suggest that occupation of the EGF receptor is required for both the mitogenic and colony-forming activity of SGF.