A Novel QTL qTGW3 Encodes the GSK3/SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice
A Novel QTL qTGW3 Encodes the GSK3/SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice
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新型 QTL qTGW3 编码 GSK3/SHAGGY 样激酶 OsGSK5/OsSK41，与 OsARF4 相互作用，负向调节水稻的籽粒大小和重量
DOI：
10.1016/j.molp.2018.03.005
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发表时间：
2018-05-07
期刊：
影响因子：
27.5
通讯作者：
Liu, Jian-Xiang
中科院分区：
文献类型：
--
作者：
Hu, Zejun;Lu, Sun-Jie;Liu, Jian-Xiang
水稻籽粒的大小和形状是决定粒重和产量的重要因素。在这里，我们报告了一个新的主要数量性状基因座（QTL），qTGW 3，控制水稻籽粒大小和重量。该基因座qTGW 3编码OsSK 41（也称为OsGSK 5），OsSK 41是糖原合成酶激酶3/SHAGGY样家族的成员。携带OsSK 41功能丧失等位基因的水稻近等基因系具有增加的粒长和粒重。我们证明OsSK 41与生长素反应因子4（OsARF 4）相互作用并使其磷酸化。OsSK 41与OsARF 4的共表达增加了OsARF 4在水稻原生质体中的积累。OsARF 4功能的丧失导致水稻籽粒变大。RNA测序分析表明，OsARF 4和OsSK 41在水稻籽粒发育过程中抑制一组共同的下游基因的表达，包括一些生长素响应基因。OsSK 41在qTGW 3处的功能丧失形式代表了尚未在水稻育种中广泛利用的罕见等位基因。通过靶向基因编辑或QTL嵌合抑制OsSK 41功能可增强水稻籽粒大小和重量。本研究揭示了OsSK 41在水稻籽粒发育中的重要作用，为水稻乃至其他禾谷类作物产量的遗传改良提供了新的候选基因。
Grain size and shape are important determinants of grain weight and yield in rice. Here, we report a new major quantitative trait locus (QTL), qTGW3, that controls grain size and weight in rice. This locus, qTGW3, encodes OsSK41 (also known as OsGSK5), a member of the GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like family. Rice near-isogenic lines carrying the loss-of-function allele of OsSK41 have increased grain length and weight. We demonstrate that OsSK41 interacts with and phosphorylates AUXIN RESPONSE FACTOR 4 (OsARF4). Co-expression of OsSK41 with OsARF4 increases the accumulation of OsARF4 in rice protoplasts. Loss of function of OsARF4 results in larger rice grains. RNA-sequencing analysis suggests that OsARF4 and OsSK41 repress the expression of a common set of downstream genes, including some auxin-responsive genes, during rice grain development. The loss-of-function form of OsSK41 at qTGW3 represents a rare allele that has not been extensively utilized in rice breeding. Suppression of OsSK41 function by either targeted gene editing or QTL pyramiding enhances rice grain size and weight. Thus, our study reveals the important role of OsSK41 in rice grain development and provides new candidate genes for genetic improvement of grain yield in rice and perhaps in other cereal crops.