NADPH oxidase immunoreactivity in the mouse brain
NADPH oxidase immunoreactivity in the mouse brain
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DOI：
10.1016/s0006-8993(03)03364-x
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发表时间：
2003-10-24
期刊：
影响因子：
2.9
通讯作者：
Klann, E
中科院分区：
文献类型：
--
作者：
Serrano, F;Kolluri, NS;Klann, E
通过NADPH氧化酶产生的超氧化物已被证明在神经毒性、缺血性中风以及可能的帕金森病和阿尔茨海默病中发挥作用。此外，NADPH氧化酶依赖的超氧化物的产生可能是正常脑功能所必需的，包括神经元分化和神经元可塑性。为了提高我们对大脑中NADPH氧化酶的认识，我们利用免疫组织化学方法研究了成年小鼠中枢神经系统中NADPH氧化酶的各种蛋白质成分的定位。我们检测了细胞质NADPH蛋白p40（phox）、p47（phox）和p67（phox）的染色，以及膜相关NADPH氧化酶蛋白p22（phox）和gp9l（phox）在整个小鼠大脑神经元中的染色。在神经轴的所有区域的神经元中观察到每一种NADPH氧化酶蛋白的染色，在海马、皮层的定位特别突出。杏仁核。纹状体和丘脑。神经元中NADPH氧化酶蛋白的表达表明，NADPH氧化酶产生超氧化物可能在正常神经元功能和大脑神经退行性变中都起作用。(C) 2003 Elsevier B.V.版权所有
Superoxide production via NADPH oxidase has been shown to play a role in neurotoxicity, ischemic stroke, and possibly Parkinson's and Alzheimer's diseases. In addition, NADPH oxidase-dependent production of superoxide may be necessary for normal brain functions, including neuronal differentiation and neuronal plasticity. To improve our understanding of NADPH oxidase in the brain, we studied the localization of the various protein components of NADPH oxidase in the central nervous system of the adult mouse using immunohistochemistry. We detected staining for the cytoplasmic NADPH proteins, p40(phox), p47(phox), and p67(phox), as well as the membrane-associated NADPH oxidase proteins, p22(phox) and gp9l(phox) in neurons throughout the mouse brain. Staining of each of the NADPH oxidase proteins was observed in neurons in all regions of the neuraxis, with particularly prominent localizations in the hippocampus, cortex. amygdala. striatum, and thalamus. The expression of NADPH oxidase proteins in neurons suggests the possibility that enzymatic production of superoxide by a NADPH oxidase may play a role in both normal neuronal function as well as neurodegeneration in the brain. (C) 2003 Elsevier B.V. All rights reserved.