Effects of endoplasmic reticulum stress on the expression of inflammatory cytokines in patients with ulcerative colitis
Effects of endoplasmic reticulum stress on the expression of inflammatory cytokines in patients with ulcerative colitis
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DOI：
10.3748/wjg.v22.i7.2357
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发表时间：
2016-02-21
影响因子：
4.3
通讯作者：
Zhao, Ya-Jiao
中科院分区：
文献类型：
--
作者：
Li, Nan;Wang, Xue-Ming;Zhao, Ya-Jiao
目的：目的：探讨溃疡性结肠炎（UC）患者内质网应激（ERS）时X-box结合蛋白1（X-box binding protein 1，XBP 1 s）剪接及炎性细胞因子表达的变化。结果：正常人和UC患者外周血单核细胞分别用无药物（对照）、植物血凝素（PHA）、毒胡萝卜素（TG）或PHA和TG联合刺激。UC患者的XBP 1显示剪接，共刺激比单刺激更大。结论：正常人和UC患者的T淋巴细胞对ERS的应答均通过激活XBP 1 s介导的信号通路，上调炎性细胞因子的表达，增加炎症的发生。UC患者外周血单个核细胞对ERS的敏感性高于正常人。
AIM: To explore the changes of X-box binding protein 1 splicing (XBP1s) and inflammatory cytokine expression in patients with ulcerative colitis (UC) in response to endoplasmic reticulum stress (ERS).METHODS: Reverse transcription polymerase chain reaction and quantitative polymerase chain reaction were performed to detect the forms of XBP1s and the expression of interleukin (IL)-2, interferon (IFN)-gamma, and IL-17 alpha. Differences between patients with UC and normal subjects were then determined.RESULTS: Mononuclear cells of the peripheral blood of normal subjects and UC patients with were stimulated with no drugs (control), phytohemagglutinin (PHA), thapsigargin (TG), or both PHA and TG. XBP1s in patients with UC exhibited splicing, which was greater with co-stimulation than single stimulation. Co-stimulation increased the expression level of IL-2, IFN-gamma, and IL-17 alpha.CONCLUSION: The T lymphocytes of both normal subjects and patients with UC responded to ERS by activating the XBP1s-mediated signalling pathway, upregulating the expression of inflammatory cytokines, and increasing the occurrence of inflammation. The mononuclear cells in the peripheral blood of patients with UC were more sensitive to ERS than those in the peripheral blood of normal subjects.