Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata
Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata
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DOI：
10.1007/s11240-007-9324-2
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Zhang, Jianxia
中科院分区：
文献类型：
--
作者：
Fan, Chaohong;Pu, Ni;Zhang, Jianxia
从中国野生葡萄（W. T. Wang）中克隆的一种新型二苯乙烯合酶基因（STS）负责葡萄树中植物抗毒素白藜芦醇的合成，并成功转移到葡萄中。 Thompson Seedless 通过根癌农杆菌介导的转化。使用本研究中开发的转化程序，产生了 72% GFP 阳性萌发胚胎，其中约 38% 的转化胚胎再生为正常植株。通过PCR和Southern印迹分析验证STS基因整合到转基因植物中。通过高效液相色谱(HPLC)检测STS基因的表达情况，结果表明转基因植株中白藜芦醇的浓度比未转化的对照植株高5.5倍。
A novel stilbene synthase gene (STS), cloned from Chinese wild Vitis pseudoreticulata (W. T. Wang) and responsible for synthesis of the phytoalexin resveratrol in grapevine, was successfully transferred into V. vinifera L. cv. Thompson Seedless via Agrobacterium tumefaciens-mediated transformation. Using transformation procedures developed in the present study, 72% GFP-positive germinated embryos were produced with about 38% of transformed embryos regenerated into normal plantlets. Integration of the STS gene into the transgenic plants was verified by PCR and Southern blot analysis. Expression of the STS gene was detected by high performance liquid chromatography (HPLC), which showed that the resveratrol concentration in the transgenic plants was 5.5 times higher than that in non-transformed control plants.