Dynamic instability of the intracellular pressure drives bleb-based motility
Dynamic instability of the intracellular pressure drives bleb-based motility
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DOI：
10.1242/jcs.065672
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发表时间：
2010-11-15
影响因子：
4
通讯作者：
Amblard, Francois
中科院分区：
文献类型：
--
作者：
Maugis, Benoit;Brugues, Jan;Amblard, Francois
我们已经证明，人类致病寄生虫溶组织内阿米巴(Eh)的二维和三维运动依赖于细胞内静水压力的持续不稳定性。这种不稳定性驱动膜泡的循环生成和愈合，典型的突出速度为10-20微米/秒，持续数百毫秒，愈合时间为10秒。使用一种新的微电穿孔方法来控制细胞内压，使我们能够建立一个带有三个参数的定性模型：肌球蛋白驱动的内压增加的速率；膜-细胞骨架键的临界分离应力；以及F-肌动蛋白皮质的周转时间。虽然气泡在空间和时间上是随机出现的，但它们可以在受限的几何空间中以定义的周期发生，从而证实了我们的模型。鉴于Eh在体外和体内基于泡状细胞的高效运动，Eh细胞代表了在二维和三维环境中研究阿米巴和间充质运动的物理和生物学方面的独特模型。
We have demonstrated that the two-and three-dimensional motility of the human pathogenic parasite Entamoeba histolytica (Eh) depends on sustained instability of the intracellular hydrostatic pressure. This instability drives the cyclic generation and healing of membrane blebs, with typical protrusion velocities of 10-20 mu m/second over a few hundred milliseconds and healing times of 10 seconds. The use of a novel micro-electroporation method to control the intracellular pressure enabled us to develop a qualitative model with three parameters: the rate of the myosin-driven internal pressure increase; the critical disjunction stress of membrane-cytoskeleton bonds; and the turnover time of the F-actin cortex. Although blebs occur randomly in space and irregularly time, they can be forced to occur with a defined periodicity in confined geometries, thus confirming our model. Given the highly efficient bleb-based motility of Eh in vitro and in vivo, Eh cells represent a unique model for studying the physical and biological aspects of amoeboid versus mesenchymal motility in two-and three-dimensional environments.