Canine arteries release two different endothelium-derived relaxing factors.
Canine arteries release two different endothelium-derived relaxing factors.
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犬动脉释放两种不同的内皮源性舒张因子。
DOI：
10.1152/ajpheart.1989.257.1.h330
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
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作者：
Hoeffner,U;Feletou,M;Flavahan,NA;Vanhoutte,PM
本实验旨在分析瓦巴因对血管平滑肌对基础条件下释放的内皮源性松弛因子的反应以及对乙酰胆碱或缓激肽刺激的影响。用带内皮的左旋冠状动脉（供动脉）的灌注液灌注犬冠状动脉（无内皮）的生物测定环。在前列腺素F2 α引起的生物测定环收缩过程中，在基础条件下或供体动脉内皮细胞暴露于最大有效浓度的乙酰胆碱时，内皮来源的松弛因子(s)引起的松弛通过瓦巴因的生物测定环孵育而减少。然而，缓激肽输注引起的松弛未被瓦巴因的生物测定环所改变。这些实验证明了两种内皮来源的松弛因子的释放，可以用瓦巴因来区分。
Experiments were designed to analyze the effects of ouabain on the response of vascular smooth muscle to endothelium-derived relaxing factors released under basal conditions and on stimulation with acetylcholine or bradykinin. Bioassay rings of canine coronary artery (without endothelium) were superfused with perfusate from canine left circumflex coronary arteries with endothelium (donor arteries). During contractions of the bioassay ring evoked by prostaglandin F2 alpha, the relaxations caused by endothelium-derived relaxing factor(s), released under basal conditions or on exposure of the endothelial cells of the donor artery to maximally effective concentrations of acetylcholine, were reduced by incubation of the bioassay ring with ouabain. However, the relaxations evoked by infusion of bradykinin were not altered by incubation of the bioassay rings with ouabain. These experiments demonstrate the release of two endothelium-derived relaxing factors that can be distinguished using ouabain.