Paracrine effect of CXCR4-overexpressing mesenchymal stem cells on ischemic heart injury.
Paracrine effect of CXCR4-overexpressing mesenchymal stem cells on ischemic heart injury.
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CXCR4-过表达间充质干细胞对缺血性心脏损伤的旁分泌作用
DOI：
10.1002/cbf.3254
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发表时间：
2017-03
影响因子：
3.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Wu SZ;Li YL;Huang W;Cai WF;Liang J;Paul C;Jiang L;Wu ZC;Xu M;Zhu P;Wang Y
It has been reported that CXCR4-overexpressing Mesenchymal stem cells (MSCCX4) can repair heart tissue post myocardial infarction (MI). This study aims to investigate the MSCCX4-derived paracrine cardio-protective signaling in the presence of MI. MSCs were divided into 3 groups: MSC only, MSCCX4, and CXCR4 gene-specific siRNA-transduced MSC (MSCsiR). MSCs were exposed to hypoxia, and then MSCs-conditioned culture medium was incubated with neonatal and adult cardiomyocytes respectively. Cell proliferation-regulating genes were assessed by RT-PCR. In vitro: The number of cardiomyocytes undergoing DNA synthesis, cytokinesis, and mitosis was increased to a greater extent in MSCCX4 medium-treated group than control group, while this pro-proliferative effect was reduced in MSCsiR-treated cells. Accordingly, the maximal enhancement of VEGF, cyclin 2, and TGF-β2 was observed in hypoxia-exposed MSCCX4. In vivo: MSCs were labeled with EGFP and engrafted into injured-myocardium in rats. The number of EGFP and CD31 positive cells in the MSCCX4 group were significantly increased than other 2 groups, associated with the reduced left ventricular (LV) fibrosis, the increased LV free wall thickness, the enhanced angiogenesis, and the improved contractile function. CXCR4 overexpression can mobilize MSCs into ischemic area, whereby these cells can promoted angiogenesis and alleviate left ventricular remodeling via paracrine signaling mechanism.
促丝分裂原后心肌细胞的分化表现出心室生长反应的三个顺序状态。
影响因子：
7.8
作者：
Ueno, H;Perryman, M B;Roberts, R;Schneider, M D
通讯作者：
Schneider, M D