Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene
Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene
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DOI：
10.1073/pnas.0702860104
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发表时间：
2007-05-22
影响因子：
11.1
通讯作者：
Tsuchida, Kozo
中科院分区：
文献类型：
--
作者：
Sakudoh, Takashi;Sezutsu, Hideki;Tsuchida, Kozo
类胡萝卜素是人类必需的营养素，其摄取和运输机制在任何动物系统中都没有得到很好的理解。家蚕黄血基因是决定蚕茧颜色的关键基因，控制着家蚕肠粘膜和丝腺对类胡萝卜素的吸收。在这里，我们提供的证据表明，Y基因对应的细胞内类胡萝卜素结合蛋白（CBP）基因。在Y隐性菌株中，可能由于转座子相关基因组缺失导致的不正确mRNA剪接导致外显子缺失，产生无功能CBP mRNA，导致无色血淋巴和白色茧。通过转CBP基因的方法，提高了白色茧对类胡萝卜素的吸收和着色能力。这项研究表明，在动物门类胡萝卜素摄取的被动扩散以外的遗传促进细胞内过程的存在，并通过基因工程为调节丝的颜色和脂质含量铺平了道路。
Mechanisms for the uptake and transport of carotenoids, essential nutrients for humans, are not well understood in any animal system. The Y(Yellow blood) gene, a critical cocoon color determinant in the silkworm Bombyx mori, controls the uptake of carotenoids into the intestinal mucosa and the silk gland. Here we provide evidence that the Y gene corresponds to the intracellular carotenoid-binding protein (CBP) gene. In the Y recessive strain, the absence of an exon, likely due to an incorrect mRNA splicing caused by a transposon-associated genomic deletion, generates a nonfunctional CBP mRNA, resulting in colorless hemolymph and white cocoons. Enhancement of carotenoid uptake and coloration of the white cocoon was achieved by germ-line transformation with the CBP gene. This study demonstrates the existence of a genetically facilitated intracellular process beyond passive diffusion for carotenoid uptake in the animal phyla, and paves the way for modulating silk color and lipid content through genetic engineering.