APPLICATION OF THE MICROPIPETTE TECHNIQUE TO THE MEASUREMENT OF CULTURED PORCINE AORTIC ENDOTHELIAL-CELL VISCOELASTIC PROPERTIES
APPLICATION OF THE MICROPIPETTE TECHNIQUE TO THE MEASUREMENT OF CULTURED PORCINE AORTIC ENDOTHELIAL-CELL VISCOELASTIC PROPERTIES
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DOI：
10.1115/1.2891183
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发表时间：
1990-08-01
影响因子：
1.7
通讯作者：
NEREM, RM
中科院分区：
文献类型：
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作者：
SATO, M;THERET, DP;NEREM, RM
使用微量移液器技术测量在静态培养条件下生长的猪主动脉内皮细胞的粘弹性变形。对对照细胞（通过机械或胰蛋白酶从基质上分离）和细胞骨架元件被细胞松弛素 B 或秋水仙碱破坏的细胞进行了实验。在逐步增加抽吸压力后，测量进入移液器的抽吸长度的时间过程。为了分析数据，使用了内皮细胞的标准线性粘弹性半空间模型。吸气长度表示为时间的指数函数。发现肌动蛋白微丝是决定对静态培养中生长的内皮细胞的粘弹性反应的主要细胞骨架成分。
The viscoelastic deformation of porcine aortic endothelial cells grown under static culture conditions was measured using the micropipette technique. Experiments were conducted both for control cells (mechanically or trypsin detached from the substrate) and for cells in which cytoskeletal elements were disrupted by cytochalasin B or colchicine. The time course of the aspirated length into the pipette was measured after applying a stepwide increase in aspiration pressure. To analyze the data, a standard linear viscoelastic half-space model of the endothelial cell was used. The aspirated length was expressed as an exponential function of time. The actin microfilaments were found to be the major cytoskeletal component determining the viscoelastic response to endothelial cells grown in static culture.