Matrix-assisted laser desorption/ionization mass spectrometry for the analysis of polyamines in plant micro-tissues using cucurbituril as a host molecule
Matrix-assisted laser desorption/ionization mass spectrometry for the analysis of polyamines in plant micro-tissues using cucurbituril as a host molecule
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使用葫芦脲作为主体分子的基质辅助激光解吸/电离质谱法分析植物微组织中的多胺
DOI：
10.1016/j.aca.2017.08.027
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发表时间：
2017-09-22
影响因子：
6.2
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Ding, Jun;Liu, Simin;Feng, Yu-Qi
提出了一种以葫芦[n]脲（CB[n]）为主体分子的基质辅助激光解吸/电离（MALDI）质谱（MS）方法，用于复杂样品中低分子量（LMW）化合物的分析。作为概念验证，CB[6]被选为宿主分子，植物组织中的内源性多胺被选为目标分析物。由于CB[6]的分子识别和质量移动特性，与多胺相关的离子信号在与CB[6]特异性结合后移动到较高质量区（>1000 Da），而来自常规有机基质和复杂样品基质的信号干扰由于其分子大小与CB[6]空腔的不相容性而保持在低质量区。该策略不仅有利于低分子量化合物在复杂的样品中的分析，通过MALDI MS，但也提供了高通量完成整个分析过程在10分钟内。多胺浓度的检测显示出良好的线性范围内的0.02-10.0 ng/mL的相关系数（R）大于0.9915。方法的检出限为8.8-28.8 pg，日内、日间精密度良好，相对标准偏差小于7.9%，回收率为92.1%~ 117.1%。最后，良好的灵敏度的方法允许在各种微组织的拟南芥（每个样品20.0-740.0毫克鲜重）的内源多胺浓度的定量分析。(C)2017爱思唯尔B. V.保留所有权利。
In this study, a matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) strategy using cucurbit[n]uril (CB[n]) as a host molecule is proposed for the analysis of low molecular weight (LMW) compounds in complex samples. As a proof-of-concept, CB[6] was selected as the host molecule, and endogenous polyamines in plant tissue were chosen as the target analytes. Due to the molecular recognition and mass shifting properties of CB[6], the ionic signals associated with polyamines were moved to the higher mass region (>1000 Da) after specifically binding to CB[6], while signal interference derived from the conventional organic matrix and the complex sample matrix remained in the low mass region because of the incompatibility of their molecular size with CB[6] cavities. The strategy not only facilitated the analysis of LMW compounds in complex samples by MALDI MS, but also offered high throughput by accomplishing the entire analytical procedure within 10 min. The detection of polyamine concentration showed good linearity in the range of 0.02-10.0 ng/mL with correlation coefficients (R) greater than 0.9915. The limits of detection were 8.8-28.8 pg. The good reproducibility and reliability of the method were demonstrated by excellent intraday and interday precisions with relative standard deviations less than 7.9%, and the recovery ranged from 92.1% to 117.1%. Finally, the good sensitivity of the method allowed for the quantitative analysis of endogenous polyamine concentrations in various microtissues of Arabidopsis thaliana (20.0-740.0 mg fresh weight for each sample). (C) 2017 Elsevier B.V. All rights reserved.