FIBROBLASTS MODULATE INTESTINAL SECRETORY RESPONSES TO INFLAMMATORY MEDIATORS
FIBROBLASTS MODULATE INTESTINAL SECRETORY RESPONSES TO INFLAMMATORY MEDIATORS
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DOI：
10.1172/jci115610
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发表时间：
1992-02-01
影响因子：
15.9
通讯作者：
POWELL, DW
中科院分区：
文献类型：
--
作者：
BERSCHNEIDER, HM;POWELL, DW
培养的结肠上皮细胞和成纤维细胞被用来检查这些细胞类型之间的相互作用，在肠道分泌。T84结肠上皮细胞的分泌反应，测定为在改良的Ussing室的缓激肽，5-羟色胺，过氧化氢和组胺的短路电流的变化，增强成纤维细胞的存在下，无论是在共培养或单独培养的成纤维细胞急性并列与T84文化。吲哚美辛预处理可消除这种作用，并发现成纤维细胞释放前列腺素E2对这些炎症介质的反应。成纤维细胞可能通过对炎症介质产生和释放环氧合酶产物，对肠上皮细胞的分泌反应进行旁分泌调节。这些研究表明，肠成纤维细胞鞘的一种新功能：通过间质/上皮相互作用放大炎症反应。
Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal secretion. Secretory responses of T84 colonic epithelial cells, measured as changes in the short-circuit current in modified Ussing chambers to bradykinin, serotonin, hydrogen peroxide, and histamine, were enhanced in the presence of fibroblasts, either in cocultures or when separate cultures of fibroblasts were acutely juxtaposed with the T84 cultures. This effect was abolished by pretreatment with indomethacin and the fibroblasts were found to release prostaglandin E2 in response to these inflammatory mediators. Fibroblasts may exert a paracrine regulation on the secretory response of intestinal epithelial cells via the generation and release of cyclooxygenase products in response to inflammatory mediators. These studies suggest a novel function for the intestinal fibroblastic sheath: that of amplification of the inflammatory response through mesenchymal/epithelial interaction.