High-throughput automated droplet microfluidic system for screening of reaction conditions
High-throughput automated droplet microfluidic system for screening of reaction conditions
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DOI：
10.1039/b925500a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Garstecki, Piotr
中科院分区：
文献类型：
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作者：
Churski, Krzysztof;Korczyk, Piotr;Garstecki, Piotr
我们展示了一种新的液滴按需（DOD）技术和一个集成的系统，用于扫描任意组合的3混溶溶液在类似于1.5 μ L液滴在3 Hz。DOD系统使用微流体芯片外部的标准电磁阀。该功能弥补了模块化，组装简单性和与几乎任何微流体芯片的兼容性，并产生小于1 mm的芯片上占用空间（2）。一种用于形成DOD的新方案使得能够在类似于30 Hz的最大操作频率下生成任意大范围体积的液滴。我们展示的集成系统可用于扫描高达10 000个条件的化学和生化反应每小时使用类似的10 mL的解决方案。
We demonstrate a new droplet on demand (DOD) technique and an integrated system for scanning of arbitrary combinations of 3 miscible solutions in similar to 1.5 mu L droplets at 3 Hz. The DOD system uses standard electromagnetic valves that are external to the microfluidic chip. This feature makes up for modularity, simplicity of assembly and compatibility with virtually any microfluidic chip and yields an on-chip footprint of less than 1 mm(2). A novel protocol for formation of DOD enables generation of an arbitrarily large range of volumes of droplets at a maximum operational frequency of similar to 30 Hz. The integrated system that we demonstrate can be used to scan up to 10 000 conditions of chemical and biochemical reactions per hour using similar to 10 mL of solutions in total.