Transient peaks in the delayed luminescence from Scenedesmus obtusiusculus induced by phosphorus starvation and carbon dioxide deficiency
Transient peaks in the delayed luminescence from Scenedesmus obtusiusculus induced by phosphorus starvation and carbon dioxide deficiency
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磷饥饿和二氧化碳缺乏诱导的钝生栅藻延迟发光的瞬时峰
DOI：
10.1111/j.1399-3054.1986.tb01912.x
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发表时间：
1986
影响因子：
6.4
通讯作者：
J. Tillberg
中科院分区：
文献类型：
--
作者：
S. Mellvig;J. Tillberg
将单细胞绿藻（Scenedesmus obtusiusculus, Chod.）的细胞饥饿24、48、72和96 h，观察不同饥饿条件下细胞延迟发光的衰减动力学。饥饿24 h的细胞在白光激发后表现出与对照细胞相似的延迟发光衰减动力学和光子累积输出。在缺磷48 h后观察到延迟发光衰减动力学的两个瞬态峰（有几个组分），而在72或96 h后没有观察到。瞬态峰的振幅取决于白光激发期的长度和光激发前暗期的长度。高CO2可用性不诱导瞬态峰值，低CO2可用性诱导高瞬态峰值。660和680 nm光激发不能产生瞬时峰，700 nm光激发只产生一个瞬时峰。当培养基pH由7.2变为9.5时，缺磷96 h的细胞和对照组的延迟发光动力学发生改变，光子累积输出减少。这些数据表明，延迟发光的瞬态峰的产生机制涉及一个复杂的代谢模式。主要因素可能是叶绿体中三糖易位的减少，卡尔文循环活性的减少以及ATP和可用还原剂量的变化。
Cells of the unicellular green alga Scenedesmus obtusiusculus (Chod.) were starved of phosphorus for 24, 48, 72 and 96 h, and the decay kinetics of the delayed luminescence from the differently starved cells was monitored for several minutes. Cells starved for 24 h showed similar delayed luminescence decay kinetics and accumulated output of photons as control cells after excitation with white light. Two transient peaks (with several components) in the decay kinetics of delayed luminescence were observed after 48 h of phosphorus starvation but not after 72 or 96 h. The amplitude of the transient peaks varied depending on the length of the excitation period with white light and on the length of the dark period preceding light excitation. High CO2 availability induced no transient peak, whereas low CO2 availability induced a high transient peak. Transient peaks could not be induced by excitation with light of 660 or 680 nm and only a single transient peak developed using 700 nm light. The kinetics of the delayed luminescence was changed, and the accumulated output of photons was decreased when the pH of the medium was changed from 7.2 to 9.5, both in cells starved for phosphorus for 96 h and in controls. The data indicate that a complicated metabolic pattern is involved in the mechanisms giving rise to the observed transient peaks in the delayed luminescence. The main factors may be a reduction in the translocation of trioses from chloroplasts, a concomitant reduction in Calvin cycle activities and changes in the amount of ATP and reducing agents available.