Dual ultrastructural immunocytochemical labeling of μ and δ opioid receptors in the superficial layers of the rat cervical spinal cord
Dual ultrastructural immunocytochemical labeling of μ and δ opioid receptors in the superficial layers of the rat cervical spinal cord
复制标题
DOI：
10.1016/s0006-8993(97)00891-3
复制
发表时间：
1997-12-19
期刊：
影响因子：
2.9
通讯作者：
Pickel, VM
中科院分区：
文献类型：
--
作者：
Cheng, PY;Liu-Chen, LY;Pickel, VM
β阿片受体(DOR)和单位阿片受体(MOR)广泛分布于脊髓背角。选择性阿片受体激动剂同时激活每个受体可产生协同止痛作用。为了确定这些功能联系的细胞学基础，我们通过成年大鼠脊髓(C2-C4；)背角浅层的单个切片，观察了DOR和MOR的电子显微镜免疫细胞化学定位。在总共270个DOR标记的轮廓中，49%是胞体和树突，46%是轴突终末和小的无髓轴突，5%是神经胶质突起。6%的DOR标记的胞体和树突以及1%的胶质突起也显示MOR样免疫反应(MOR-LI)。在339个MOR标记的轮廓中，87%是轴突终末和小的无髓轴突，12%是胞体和树突，2%是神经胶质突起。21%的MOR标记的胞体和树突，但没有轴突终末也含有DOR-LI。MOR和DOR在轴突终末的亚细胞分布是不同的。在轴突终末，DOR-LI和MOR-LI均沿质膜分布，但仅DOR-LI与大而致密的核心小泡有关。DOR标记的终末与含有MOR的树突形成突触，反之，MOR标记的终末与DOR标记的树突形成突触。这些结果表明，选择性MOR和DOR激动剂的协同作用可能归因于脊髓浅层相同或突触联系的神经元的双重调制。(C)1997年爱思唯尔科学公司。
The delta opioid receptor (DOR) and mu opioid receptor (MOR) are abundantly distributed in the dorsal horn of the spinal cord. Simultaneous activation of each receptor by selective opiate agonists has been shown to result in synergistic analgesic effects. To determine the cellular basis for these functional associations, we examined the electron microscopic immunocytochemical localization of DOR and MOR in single sections through the superficial layers of the dorsal horn in the adult rat spinal cord (C2-C4;). From a total of 270 DOR-labeled profiles, 49% were soma and dendrites, 46% were axon terminals and small unmyelinated axons, and 5% were glial processes. 6% of the DOR-labeled soma and dendrites, and < 1% of the glial processes also showed MOR-like immunoreactivity (MOR-LI). Of 339 MOR-labeled profiles, 87% were axon terminals and small unmyelinated axons, 12% were soma and dendrites, and 2% were glial processes. 21% of the MOR-labeled soma and dendrites, but none of the axon terminals also contain DOR-LI. The subcellular distributions of MOR and DOR were distinct in axon terminals. In axon terminals, both DOR-LI and MOR-LI were detected along the plasmalemma, but only DOR-LI was associated with large dense core vesicles. DOR-labeled terminals formed synapses with dendrites containing MOR and conversely, MOR-labeled terminals formed synapses with DOR-labeled dendrites. These results suggest that the synergistic actions of selective MOR- and DOR-agonists may be attributed to dual modulation of the same or synaptically linked neurons in the superficial layers of the spinal cord. (C) 1997 Elsevier Science B.V.