Direct relationship between high-energy phosphate content and blood flow in thermally treated murine tumors.
Direct relationship between high-energy phosphate content and blood flow in thermally treated murine tumors.
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DOI：
10.1093/jnci/75.5.885
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发表时间：
1985-11
期刊：
影响因子：
--
通讯作者：
M. Lilly;C. Katholi;T. Ng
中科院分区：
文献类型：
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作者：
M. Lilly;C. Katholi;T. Ng
在体内31 P-核磁共振（NMR）光谱和133氚清除分别用于连续监测ATP含量和血流量，在C3 H/HeJ小鼠皮下RIF-1肿瘤热处理。ATP迅速降低[通过光谱法测量，并表示为ATP与Pi的比值（ATP/Pi）]。ATP变化的斜率和幅度与肿瘤血流的斜率和幅度密切相关。当按给药组和个体小鼠分析数据时，观察到这两个变量之间密切相关。结扎的肿瘤在ATP/Pi和血流方面表现出定性和定量相似的变化。在体外加热到类似程度的RIF-1细胞显示ATP没有减少。在体内加热的皮下RIF-1肿瘤中ATP的损失主要是由于肿瘤血流的破坏。这些数据强调了血管因素对体内热效应的重要性。体内31 P-NMR光谱可用于间接监测高温对血管的影响。
In vivo 31P-nuclear magnetic resonance (NMR) spectroscopy and 133Xe clearance were used to monitor serially ATP content and blood flow, respectively, in C3H/HeJ mouse subcutaneous RIF-1 tumors treated with hyperthermia. There was a prompt decrease in ATP [measured spectroscopically and expressed as the ratio of ATP to Pi (ATP/Pi)]. The slope and magnitude of the change in ATP closely paralleled those of tumor blood flow. Close correlation between these two variables was seen when data were analyzed both by treatment group and by individual mouse. Ligated tumors showed qualitatively and quantitatively similar changes in ATP/Pi and blood flow. RIF-1 cells heated in vitro to a similar degree showed no decrease in ATP. The loss of ATP in subcutaneous RIF-1 tumors heated in vivo was primarily due to disruption of tumor blood flow. These data emphasize the importance of vascular factors to in vivo thermal effects. In vivo 31P-NMR spectroscopy can be used to monitor indirectly vascular effects from hyperthermia.