Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain
Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain
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DOI：
10.1016/j.cbpa.2006.02.003
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发表时间：
2006-05-01
影响因子：
2.3
通讯作者：
Lin, Hui-Chen
中科院分区：
文献类型：
--
作者：
Chung, Kuei-Fang;Lin, Hui-Chen
Scylla paramosain是一种能主动调节其血淋巴渗透压的广盐性海蟹。我们研究了Na， k - atp酶在渗透调节器官中的表达，包括前鳃，后鳃，触角腺和中肠。我们对Na， k - atp酶α亚基的cDNA测序显示，在研究的四种渗透调节器官中都没有同种异构体。cDNA全长3866 bp，编码1039个氨基酸的完整开放阅读框。血淋巴的渗透压在转移后立即由25 ppt变为5和45 ppt，并在3天内达到新的稳定状态，表现出典型的弱高渗调节剂模式。测定在5、25和45 ppm （ppt）环境下驯化7天的个体Na、k - atp酶α亚基mRNA的表达水平。与25 ppt时相比，5 ppt时后鳃mRNA水平显著升高。45 ppt的前鳃、后鳃和触角腺的mRNA水平显著高于25 ppt的各器官。5 ppt处理的增加可能是第14天观察到的Na， k - atp酶活性增加的原因，当时后鳃的活性显著高于其他两种盐度处理。不同盐度的蛋白质水平没有差异。综上所述，后鳃在稀释海水中的渗透调节中发挥了最重要的作用，第7天Na， k - atp酶活性的增加可能与基因转录和/或mRNA翻译有关。(c) 2006爱思唯尔公司版权所有。
Scylla paramamosain is a euryhaline marine crab that can actively regulate its osmotic pressure of the hemolymph. We investigated the expression of Na,K-ATPase in the osmoregulatory organs, including anterior gills, posterior gills, antennal glands, and midgut. Our cDNA sequencing of the Na,K-ATPase alpha-subunit revealed no isoforms in any of the four osmoregulatory organs studied. The full-length cDNA contains 3866 bp that encode a complete open reading frame of 1039 amino acids. The osmotic pressure of the hemolymph changed immediately after transference from 25 ppt to 5 and 45 ppt, and reached a new steady state within 3 days, showing the typical pattern of weak hyperosmoregulators. Expression of Na,K-ATPase alpha-subunit mRNA level was determined for individuals acclimated in 5, 25, and 45 parts per thousand (ppt) for 7 days. Compared to the mRNA level of those from 25 ppt, the level in the posterior gills from 5 ppt was significantly higher. Also, the mRNA levels in the anterior gills, posterior gills and antennal glands from 45 ppt were significantly higher than that of the respective organs in 25 ppt. This increase in the 5 ppt treatment may be responsible for the observed increase of Na,K-ATPase activity on day 14 when the posterior gills had a significantly higher activity than those in the other two salinity treatments. No difference was found in the protein level across salinities. In conclusion, the posterior gills play the most important role in osmoregulation in dilute seawater and the increase in Na,K-ATPase activity on the 7th day may be due to its gene transcription and/or mRNA translation. (c) 2006 Elsevier Inc. All rights reserved.