Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes
Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes
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DOI：
10.1073/pnas.0408358102
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发表时间：
2005-03-08
影响因子：
11.1
通讯作者：
Marshall, WF
中科院分区：
文献类型：
--
作者：
Stolc, V;Samanta, MP;Marshall, WF
纤毛和鞭毛在人类疾病中发挥的重要作用迫切需要鉴定参与纤毛组装和功能的基因。莱茵衣藻鞭毛再生过程中鞭毛编码基因的强烈和特异性诱导表明，这些细胞的转录谱将揭示新的鞭毛相关基因。我们已经进行了一个全基因组的RNA转录水平的分析，在鞭毛再生的衣原体通过使用无掩模光刻法产生的DNA寡核苷酸微阵列与独特的探针序列的所有外显子的19,803个预测的基因。这一分析代表了以前在这一重要模式生物中未表征的全基因组转录活性谱研究。对强诱导基因的分析揭示了大量已知的鞭毛成分，并确定了许多重要的疾病相关蛋白与纤毛和鞭毛有关，包括斑马鱼多囊肾基因Qilin，Reptin和Pontin，以及睾丸表达的tubby样蛋白TULP 2。
The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in Chlamydomonas reinhardtii suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in Chlamydomonas by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes Qilin, Reptin, and Pontin, as well as the testis-expressed tubby-like protein TULP2.