Localization and production of novel L-asparaginase from Pectobacterium carotovorum MTCC 1428
Localization and production of novel L-asparaginase from Pectobacterium carotovorum MTCC 1428
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DOI：
10.1016/j.procbio.2009.09.011
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Pakshirajan, Kannan
中科院分区：
文献类型：
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作者：
Kumar, Sanjay;Dasu, Veeranki Venkata;Pakshirajan, Kannan
细菌L-门冬酰胺酶已被广泛用于治疗各种淋巴细胞白血病。利用胡萝卜噬果胶杆菌MTCC 1428进行了新的不含精氨酸酶的L-天冬酰胺酶的定位和生产研究。利用细胞分级分离技术对L-天冬酰胺酶进行了定位。在含有L-天冬酰胺和L-天冬酰胺与葡萄糖混合的培养基上生长的胡萝卜素A MTCC 1428细胞质中，L-天冬酰胺酶的活性分别为85%和77%。在所测试的碳源中，发现L-天冬酰胺或L-天冬酰胺和葡萄糖的组合是最适合的碳源，以最大限度地提高L-天冬酰胺酶的产量。当L-天冬酰胺和葡萄糖浓度分别为4和2 g/l时，L-天冬酰胺酶的最大产量为14.56 U/ml（26.92 U/mg蛋白质）。酵母提取物、L-天冬酰胺和蛋白胨对L-天冬酰胺酶的产生有显著的影响。胡萝卜腐食假单胞菌MTCC 1428已经同化了L-天冬酰胺作为使L-天冬酰胺酶的生产最大化的必需碳源。(C)2009爱思唯尔有限公司版权所有。
Bacterial L-asparaginase has been widely used as therapeutic agent in the treatment of various lymphoblastic leukemia diseases. Studies on localization and production of novel glutaminase-free L-asparaginase were performed using Pectobacterium carotovorum MTCC 1428. The localization of L-asparaginase was carried out using cell fractionation techniques. The activity of L-asparaginase was found to be 85 and 77% in the cytoplasm of A carotovorum MTCC 1428 grown on medium containing L-asparagine and combination of L-asparagine and glucose respectively. Among the tested carbon sources, L-asparagine or the combination of L-asparagine and glucose was found to be the most suitable carbon sources to maximize the production of L-asparaginase. The maximum production of L-asparaginase was observed to be 14.56 U/ml (26.92 U/mg of protein) at 4 and 2 g/l of L-asparagine and glucose respectively. Yeast extract, L-asparagine and peptone have shown significant effect on the production of L-asparaginase. P. carotovorum MTCC 1428 has assimilated L-asparagine as an essential carbon source for maximizing the production of L-asparaginase. (C) 2009 Elsevier Ltd. All rights reserved.