MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA IN TERM AND PRETERM PARTURITION - EFFECT OF MICROBIAL INVASION OF THE AMNIOTIC CAVITY
MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA IN TERM AND PRETERM PARTURITION - EFFECT OF MICROBIAL INVASION OF THE AMNIOTIC CAVITY
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DOI：
10.1111/j.1600-0897.1994.tb01101.x
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发表时间：
1994-09-01
影响因子：
3.6
通讯作者：
COTON, DB
中科院分区：
文献类型：
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作者：
ROMERO, R;GOMEZ, R;COTON, DB
问题：本研究旨在确定：(1) 胎龄、分娩和羊膜腔微生物侵入 (MIAC) 是否与羊水中免疫反应性巨噬细胞炎症蛋白 1 α 浓度的变化相关； (2)羊水中巨噬细胞炎症蛋白1α的浓度与白细胞计数和羊水中白细胞介素8的浓度相关。方法：通过羊膜穿刺术抽取126例患者的羊水。 54 名早产且胎膜完整的女性（足月时无 MIAC 分娩，N = 21；无 MIAC 早产，N = 16；MIAC 早产，N = 17）； 62 名足月患者（无临产，N = 19；临产-无 MIAC，N = 20；临产-MIAC，N = 23）；以及10名处于妊娠中期的患者。培养羊水中的需氧菌、厌氧菌和支原体。使用经羊水验证的免疫测定法测定羊水巨噬细胞炎症蛋白 1 α 和白细胞介素 8（灵敏度：分别为 14.2 pg/ml 和 0.3 ng/ml）。对截尾数据进行 Kruskal-Wallis 方差分析 (ANOVA)、Mann-Whitney U 检验和 Spearman 等级相关进行分析。 结果：1) 仅 31.0% (9/29) 的非临产患者（中期和足月）羊水巨噬细胞炎症蛋白 1 α 存在。 2) 早产和 MIAC 患者的羊水中巨噬细胞炎症蛋白 1 α 浓度高于无 MIAC 患者（足月无 MIAC 分娩：中位 0.0 pg/ml，范围 0.0-221.2；无 MIAC 早产：中位 37.4 pg/ml，范围 0.0-494.6；MIAC 早产：中位 7171.0 pg/ml，范围 402.5-37994.0；
PROBLEM: This study was conducted to determine whether: (1) gestational age, parturition, and microbial invasion of the amniotic cavity (MIAC) are associated with changes in amniotic fluid concentrations of immunoreactive macrophage inflammatory protein-1 alpha; (2) amniotic fluid concentrations of macrophage inflammatory protein-1 alpha are correlated with the white blood cell count and the concentrations of interleukin-8 in amniotic fluid.METHOD: Amniotic fluid was retrieved by amniocentesis from 126 patients; 54 women with preterm labor and intact membranes (no MIAC-delivery at term, N = 21; no MIAC-preterm delivery, N = 16; MIAC-preterm delivery, N = 17); 62 patients at term (no labor, N = 19; labor-no MIAC, N = 20; labor-MIAC, N = 23); and 10 patients in the midtrimester of pregnancy. Amniotic fluid was cultured for aerobic, anaerobic and Mycoplasma species. Determinations of amniotic fluid macrophage inflammatory protein-1 alpha and interleukin-8 were performed with immunoassays validated for amniotic fluid (sensitivity: 14.2 pg/ml and 0.3 ng/ml, respectively). Kruskal-Wallis analysis of variance (ANOVA) for censored data, Mann-Whitney U test and Spearman's rank correlation were performed for analysis.RESULTS: 1) Amniotic fluid macrophage inflammatory protein-1 alpha was present in only 31.0% (9/29) of patients not in labor (midtrimester and term). 2) Patients with preterm labor and MIAC had higher amniotic fluid concentrations of macrophage inflammatory protein-1 alpha than those without MIAC (no MIAC-delivery at term: median 0.0 pg/ml, range 0.0-221.2; no MIAC-preterm delivery: median 37.4 pg/ml, range 0.0-494.6; MIAC-preterm delivery: median 7171.0 pg/ml, range 402.5-37994.0; P