Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering.
Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering.
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DOI：
10.3390/ma4030527
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发表时间：
2011-03-01
期刊：
影响因子：
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通讯作者：
Chiellini F
中科院分区：
文献类型：
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作者：
Mota C;Puppi D;Dinucci D;Errico C;Bártolo P;Chiellini F
本研究的目的是开发由排列的聚己内酯(ε)微丝和电纺聚乳酸-乙醇酸(PLGA)纤维组成的三维结构的双尺度支架。采用基于熔融挤出的添加剂制造技术，生产了由直径约365μm、丝间距约191μm的多层平行微细纤维(0/90°铺设图案)组成的PCL结构。聚乳酸电纺纤维的直径约为1μm，聚集在不同厚度的聚乳酸结构的顶部，显示出一定程度的排列。采用MC3T3小鼠成骨前细胞系进行细胞培养实验，结果显示细胞活力良好，在双尺度支架上具有良好的黏附性。特别是，电纺纤维对细胞形态和行为的影响是明显的，并在为细胞在细丝间隙中的定植创造了结构桥梁。
This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap.