Development of a blood oxygenation phantom for photoacoustic tomography combined with online pO2 detection and flow spectrometry
Development of a blood oxygenation phantom for photoacoustic tomography combined with online pO2 detection and flow spectrometry
复制标题
DOI：
10.1117/1.jbo.24.12.121908
复制
发表时间：
2019-12-01
影响因子：
3.5
通讯作者：
Brunker, Joanna
中科院分区：
文献类型：
--
作者：
Gehrung, Marcel;Bohndiek, Sarah E.;Brunker, Joanna
光声断层成像（PAT）对体内血氧饱和度（sO（2））具有固有的敏感性。然而，在不了解组织和仪器相关校正因子的情况下进行精确的sO（2）测量是极具挑战性的。我们已经开发了一种低成本的流动幻影，以促进PAT系统的验证。该体模由部分嵌入组织模拟材料内的管路流路组成，独立传感器提供光学吸收光谱和管中氧分压的在线监测。我们首先使用两种经常用于光声成像的小分子染料：亚甲蓝和吲哚菁绿色来测试流动体模。然后，我们证明了使用回路中血液的化学充氧和脱氧来评估sO（2）的体模的潜力。使用这种动态评估的光声sO（2）的测量在phantomy与地面真相，我们探讨的影响，多光谱处理和光谱着色的sO（2）的准确评估。未来的研究可以利用这种低成本的动态流体模来验证注量校正算法，并探索其他血液参数，如pH值以及不同液体的吸收和其他特性。(C)作者。由SPIE在知识共享署名4.0未移植许可下发布。
Photoacoustic tomography (PAT) is intrinsically sensitive to blood oxygen saturation (sO(2)) in vivo. However, making accurate sO(2) measurements without knowledge of tissue- and instrumentation-related correction factors is extremely challenging. We have developed a low-cost flow phantom to facilitate validation of PAT systems. The phantom is composed of a flow circuit of tubing partially embedded within a tissue-mimicking material, with independent sensors providing online monitoring of the optical absorption spectrum and partial pressure of oxygen in the tube. We first test the flow phantom using two small molecule dyes that are frequently used for photoacoustic imaging: methylene blue and indocyanine green. We then demonstrate the potential of the phantom for evaluating sO(2) using chemical oxygenation and deoxygenation of blood in the circuit. Using this dynamic assessment of the photoacoustic sO(2) measurement in phantoms in relation to a ground truth, we explore the influence of multispectral processing and spectral coloring on accurate assessment of sO(2). Future studies could exploit this low-cost dynamic flow phantom to validate fluence correction algorithms and explore additional blood parameters such as pH and also absorptive and other properties of different fluids. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.