Differential metal selectivity and gene expression of two zinc transporters from rice
Differential metal selectivity and gene expression of two zinc transporters from rice
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DOI：
10.1104/pp.103.026815
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发表时间：
2003-09-01
期刊：
影响因子：
7.4
通讯作者：
Schachtman, DP
中科院分区：
文献类型：
--
作者：
Ramesh, SA;Shin, R;Schachtman, DP
锌是多种生理和生化过程的必需矿物质。为了了解锌在谷物中的转运，我们确定了基因数据库中假定的锌转运蛋白。从水稻（Oryza sativa）中鉴定并鉴定了三个全长cDNA。两个cDNA部分互补的酵母（酿酒酵母）突变体在低浓度锌吸收不足。这两种转运蛋白在功能上表现出许多相似之处，但在离子选择性和活性最佳pH值方面存在差异。两种基因的表达模式也不同。其中一个基因在所有条件下都广泛表达，另一个基因主要受缺锌诱导，在根中的表达水平高于叶。虽然这两个基因的表达时间不同，但在根中表达的定位重叠。通过对这两种转运蛋白的蛋白质序列、离子选择性和基因表达模式的比较，推测它们可能在整个植物的生理过程中发挥不同的作用。
Zinc is an essential mineral for a wide variety of physiological and biochemical processes. To understand zinc transport in cereals, we identified putative zinc transporters in gene databases. Three full-length cDNAs were identified and characterized from rice (Oryza sativa). Two of the cDNAs partially complemented a yeast (Saccharomyces cerevisiae) mutant deficient in zinc uptake at low concentrations. The two transporters showed many similarities in function but differed in ionic selectivity and pH optimum of activity. Expression patterns also differed between the two genes. One gene was broadly expressed under all conditions, and the other gene was mainly induced by zinc deficiency to higher levels in roots than in leaves. Although the timing of expression differed between the two genes, localization of expression overlapped in roots. Comparisons of the protein sequences, ionic selectivity, and gene expression patterns of the two transporters suggest that they may play different roles in the physiology of the whole plant.